SNX16 negatively regulates the migration and tumorigenesis of MCF-7 cells

IF 4 Q2 CELL & TISSUE ENGINEERING Cell Regeneration Pub Date : 2013-01-01 DOI:10.1186/2045-9769-2-3

Leilei Zhang , Dajiang Qin , Chunfang Hao , Xiaodong Shu , Duanqing Pei

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引用次数: 9

Abstract

Background

Sorting nexins are a large family of proteins that are associated with various components of the endosome system and they play many roles in processes such as endocytosis, intracellular protein trafficking and cell signaling. The subcellular distribution patterns of many of them remain controversial and their in vivo functions have not been characterized yet.

Results

We investigated the subcellular distribution and function of SNX16 in this study. SNX16 is detected on Rab5-positive endosomes localized adjacent to focal adhesions at cell cortex. Inhibition of SNX23, polymerization of microtubule filaments as well as the PI3-kinase all disrupt the cell cortex distribution of SNX16. Ectopic expression of SNX16 reduces the migration and the tumor formation activity of MCF-7 cells.

Conclusion

Our results indicate that, in addition to the PI3P, there is a SNX23- and microtubule-dependent cargo transport pathway required for the proper subcellular distribution of SNX16. SNX16 plays a negative regulatory role during cell migration and tumorigenesis.

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SNX16负向调控MCF-7细胞的迁移和肿瘤发生

分选连接蛋白是一个与内体系统的各种成分相关的蛋白质大家族，它们在内吞作用、细胞内蛋白质运输和细胞信号传导等过程中发挥着许多作用。其中许多的亚细胞分布模式仍有争议，其体内功能尚未表征。结果研究了SNX16的亚细胞分布和功能。SNX16在细胞皮质局灶粘连附近的rab5阳性核内体上检测到。SNX23的抑制、微管细丝的聚合以及pi3激酶都会破坏SNX16在细胞皮层的分布。SNX16的异位表达降低了MCF-7细胞的迁移和肿瘤形成活性。结论我们的研究结果表明，除了PI3P外，SNX16的亚细胞分布还需要SNX23和微管依赖的货物运输途径。SNX16在细胞迁移和肿瘤发生过程中起负调控作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine

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