D Iu Riazantsev, P E Drobiazina, S V Hlgatian, S K Zavriev, E V Svirshchevskaia
{"title":"[[Expression of house dust mite allergens Der f1 and Der f2 in Nicotiana benthamiana leaves].","authors":"D Iu Riazantsev, P E Drobiazina, S V Hlgatian, S K Zavriev, E V Svirshchevskaia","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The class E immunoglobulins (IgE) is known to recognize conformational epitopes and therefore the native conformation of recombinant allergens is essential for their using in test-systems. Recombinant Dermatophagoides farinae house dust mite (HDM) allergens Der f1 and Der f2 were expressed in bacteria Escherichia coli and Nicotiana benthamiana plants. It has been shown that IgE in sera from children allergic to HDM recognizes Der f2 expressed both in E. coli and N. benthamiana. Mature form of Der f1 expressed in E. coli does not interact with IgE while the protein purified from N. benthamiana is able to recognize IgE as a native allergen.</p>","PeriodicalId":9325,"journal":{"name":"Bioorganicheskaia khimiia","volume":"40 4","pages":"468-78"},"PeriodicalIF":0.0000,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioorganicheskaia khimiia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The class E immunoglobulins (IgE) is known to recognize conformational epitopes and therefore the native conformation of recombinant allergens is essential for their using in test-systems. Recombinant Dermatophagoides farinae house dust mite (HDM) allergens Der f1 and Der f2 were expressed in bacteria Escherichia coli and Nicotiana benthamiana plants. It has been shown that IgE in sera from children allergic to HDM recognizes Der f2 expressed both in E. coli and N. benthamiana. Mature form of Der f1 expressed in E. coli does not interact with IgE while the protein purified from N. benthamiana is able to recognize IgE as a native allergen.
众所周知，E类免疫球蛋白（IgE）能识别构象表位，因此重组过敏原的原生构象对其在测试系统中的应用至关重要。重组 Dermatophagoides farinae 屋尘螨（HDM）过敏原 Der f1 和 Der f2 是在大肠杆菌和烟草植物中表达的。研究表明，对 HDM 过敏的儿童血清中的 IgE 可识别在大肠杆菌和烟草中表达的 Der f2。在大肠杆菌中表达的成熟形式的 Der f1 与 IgE 没有相互作用，而从 N. benthamiana 中纯化的蛋白质能够识别作为本地过敏原的 IgE。
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