Ursolic acid nanoparticles for glioblastoma therapy

IF 4.7 4区医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-06-01 DOI:10.1016/j.nano.2023.102684

Yong Li MD , Linyao Zhao MSc , Qingyu Zhao MSc, Youdong Zhou MD, Long Zhou MD, Ping Song MD, Baohui Liu PhD, Qianxue Chen PhD, Gang Deng MD

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引用次数: 1

Abstract

Background

Glioblastoma multiforme (GBM) is the most common and fatal primary tumor in the central nervous system (CNS). The effect of chemotherapy of GBM is limited due to the existence of blood-brain barrier (BBB). The aim of this study is to develop self-assembled nanoparticles (NPs) of ursolic acid (UA) for GBM treatment.

Methods

UA NPs were synthesized by solvent volatilization method. Western blot analysis fluorescent staining and flow cytometry were launched to explore the anti-glioblastoma mechanism of UA NPs. The antitumor effects of UA NPs were further confirmed in vivo using intracranial xenograft models.

Results

UA were successfully prepared. In vitro, UA NPs could significantly increase the protein levels of cleaved-caspase 3 and LC3-II to strongly eliminate glioblastoma cells through autophagy and apoptosis. In the intracranial xenograft models, UA NPs could further effectively enter the BBB, and greatly improve the survival time of the mice.

Conclusions

We successfully synthesized UA NPs which could effectively enter the BBB and show strong anti-tumor effect which may have great potential in the treatment of human glioblastoma.

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熊果酸纳米颗粒用于胶质母细胞瘤治疗

多形性胶质母细胞瘤(GBM)是中枢神经系统(CNS)最常见和最致命的原发肿瘤。由于血脑屏障(BBB)的存在，对GBM的化疗效果有限。本研究的目的是开发熊果酸(UA)自组装纳米颗粒(NPs)用于治疗GBM。方法采用溶剂挥发法合成sua NPs。采用Western blot、荧光染色、流式细胞术等方法探讨UA NPs抗胶质母细胞瘤的作用机制。通过颅内异种移植模型进一步证实了UA NPs的体内抗肿瘤作用。结果制备成功。在体外，UA NPs可以显著提高裂解caspase 3和LC3-II的蛋白水平，通过自噬和凋亡的方式强烈清除胶质母细胞瘤细胞。在颅内异种移植模型中，UA NPs能进一步有效进入血脑屏障，大大提高小鼠的存活时间。结论我们成功合成了能有效进入血脑屏障并具有较强抗肿瘤作用的UA NPs，在治疗人胶质母细胞瘤方面具有很大的潜力。

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来源期刊

Nanomedicine: Nanotechnology, Biology and Medicine 医学-纳米科技

CiteScore

8.10

自引率

3.60%

发文量

104

审稿时长

4.6 months

期刊介绍： Nanomedicine: Nanotechnology, Biology and Medicine (NBM) is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.