{"title":"Determination of Mycotoxin Contamination Levels in Rice and Dietary Exposure Assessment.","authors":"Jose Troestch, Stephany Reyes, Aracelly Vega","doi":"10.1155/2022/3596768","DOIUrl":null,"url":null,"abstract":"<p><p>The contamination by aflatoxins, ochratoxin A, and zearalenone of samples of paddy and polished rice stored in silos located in Chiriquí, Panama, was evaluated. A total of 23 samples were extracted using immunoaffinity columns and analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) and post-column photochemical derivatization. For the method used, the detection limits were lower than 0.25 <i>μ</i>g/Kg for aflatoxins (AFB<sub>1</sub>, AFB<sub>2</sub>, AFG<sub>1</sub>, AFG<sub>2</sub>) and ochratoxin A and 9.35 <i>μ</i>g/Kg for zearalenone; the limits of quantification were between 0.25 and 18.75 <i>μ</i>g/Kg, respectively. Of the samples analyzed, all of the paddy rice samples were positive for at least one of the mycotoxins studied, zearalenone being the one found with the highest incidence (90.91%); for the polished rice samples, the mycotoxin with the highest incidence was zearalenone (50%), although in concentrations lower than those established in European legislation (100 <i>μ</i>g/Kg). The estimate of the daily zearalenone intake according to the concentrations found was always less than 0.07 <i>μ</i>g/Kg/bw. This is the first report on the determination of 6 mycotoxins in rice grains from Panama by the HPLC-FLD methodology. Considering the high incidence of mycotoxins in the analyzed rice samples, regular control in the production process is recommended to improve quality and ascertain its safety.</p>","PeriodicalId":17421,"journal":{"name":"Journal of Toxicology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463030/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2022/3596768","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The contamination by aflatoxins, ochratoxin A, and zearalenone of samples of paddy and polished rice stored in silos located in Chiriquí, Panama, was evaluated. A total of 23 samples were extracted using immunoaffinity columns and analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector (FLD) and post-column photochemical derivatization. For the method used, the detection limits were lower than 0.25 μg/Kg for aflatoxins (AFB1, AFB2, AFG1, AFG2) and ochratoxin A and 9.35 μg/Kg for zearalenone; the limits of quantification were between 0.25 and 18.75 μg/Kg, respectively. Of the samples analyzed, all of the paddy rice samples were positive for at least one of the mycotoxins studied, zearalenone being the one found with the highest incidence (90.91%); for the polished rice samples, the mycotoxin with the highest incidence was zearalenone (50%), although in concentrations lower than those established in European legislation (100 μg/Kg). The estimate of the daily zearalenone intake according to the concentrations found was always less than 0.07 μg/Kg/bw. This is the first report on the determination of 6 mycotoxins in rice grains from Panama by the HPLC-FLD methodology. Considering the high incidence of mycotoxins in the analyzed rice samples, regular control in the production process is recommended to improve quality and ascertain its safety.
期刊介绍:
Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.