Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field.

Pacific journal of health (Stockton, Calif.) Pub Date : 2021-01-01 DOI:10.56031/2576-215x.1010

Zoe Ingram, Abha Patkar, Dahoon Oh, Kevin K Zhang, Christina Chung, Joan Lin-Cereghino, Geoff P Lin-Cereghino

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Abstract

The yeast Pichia pastoris (also known as Komagataella pastoris) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. In order to provide those working in this field with strategies to overcome these common obstacles, nine experts in P. pastoris protein expression field were interviewed to create a written review and video (https://www.youtube.com/watch?v=Q1oD6k8CdG8). This review describes how each respected scientist addressed a specific challenge, such as identifying high expression strains, improving secretion efficiency and decreasing hyperglycosylation. Their perspective and practical advice can be a tool to help empower others to express challenging proteins in this popular recombinant host.

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克服酵母 Pichia pastoris 蛋白质表达中的障碍：Pichia 领域领军人物访谈录》（Overcoming Obstacles in the Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field）。

Pichia pastoris（又称 Komagataella pastoris）酵母 30 多年来一直被用于生产成千上万种有价值的异源蛋白，如治疗糖尿病的胰岛素和预防偏头痛的抗体。尽管酵母取得了成功，但研究科学家在尝试使用酵母生产重组蛋白质时，还是会遇到一些常见的顽固问题。为了向这一领域的研究人员提供克服这些常见障碍的策略，我们采访了九位P. pastoris蛋白表达领域的专家，并制作了一份书面综述和视频（https://www.youtube.com/watch?v=Q1oD6k8CdG8）。这篇评论介绍了每位德高望重的科学家如何应对特定的挑战，如确定高表达菌株、提高分泌效率和减少高糖基化。他们的观点和实用建议可以作为一种工具，帮助其他人在这种流行的重组宿主中表达具有挑战性的蛋白质。

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Pacific journal of health (Stockton, Calif.)

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