Population Structure of Stagonosporopsis Species Associated with Cucurbit Gummy Stem Blight in Korea.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2022-10-01 DOI:10.5423/PPJ.OA.07.2022.0102
Yong-Jik Jeong, Oh-Kyu Kwon, A-Ram Jeong, Hyunji Lee, Hyeran Moon, O New Lee, Jeum Kyu Hong, Chang-Jin Park
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Abstract

Gummy stem blight (GSB), a common and serious disease in cucurbits worldwide, is caused by three genetically distinct species: Stagonosporopsis cucurbitacearum (syn. Didymella bryoniae), S. citrulli, and S. caricae. In Korea, however, the three species of Stagonosporopsis have been barely characterized. In this study, 21 Stagonosporopsis isolates were recovered from watermelon (Citrullus lanatus) and muskmelon (Cucumis melo) leaves and stem showing blight symptoms collected from 43 fields in Korea. Sequence analysis performed with an internal transcribed spacer region was not competent to differentiate the Stagonosporopsis isolates. On the contrary, analysis of β-tubulin (TUB) genes and three microsatellite markers, Db01, Db05, and Db06, successfully differentiated Stagonosporopsis isolates. Further sequence analysis identified two Stagonosporopsis species, S. citrulli and S. caricae, and one previously unknown species of Stagonosporopsis. Representative isolates from three species caused dark water-soaked lesions on the detached watermelon and muskmelon leaves with no significant differences in the aggressiveness. Our results indicate that the S. citrulli, S. caricae, and unknown Stagonosporopsis sp. are all causal agents of GSB for both watermelon and muskmelon. This is the first report of a new species and the population structure of Stagonosporopsis species causing GSB in Korea.

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韩国葫芦粘茎疫病相关的Stagonosporopsis种群结构。
粘茎枯萎病(GSB)是一种常见的严重疾病,在世界范围内的葫芦,是由三种遗传上不同的物种引起的:stonosporopsis cucurbitacearum(同:Didymella bryoniae), S. citrulli和S. caricae。但是,在韩国,几乎没有发现这三种。本研究从国内43个大田的西瓜(Citrullus lanatus)和甜瓜(Cucumis melo)的叶和茎中分离出21株Stagonosporopsis菌株。用内部转录间隔区进行的序列分析无法区分Stagonosporopsis菌株。相反,通过β-微管蛋白(β-tubulin, TUB)基因和3个微卫星标记Db01、Db05和Db06的分析,成功区分了Stagonosporopsis菌株。进一步的序列分析鉴定出两个Stagonosporopsis种,S. citrulli和S. caricae,以及一个以前未知的Stagonosporopsis种。3种典型菌株在离体西瓜和甜瓜叶片上产生暗色水浸病损,其侵袭性无显著差异。结果表明,西瓜和甜瓜的葡萄球菌(S. citrulli)、葡萄球菌(S. caricae)和未知的Stagonosporopsis sp.都是GSB的致病因子。这是国内首次报道引起GSB的Stagonosporopsis种的新种和种群结构。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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