Preparation of a pH-responsive chitosan-montmorillonite-nitrogen-doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Engineering in Life Sciences Pub Date : 2022-09-13 DOI:10.1002/elsc.202200016
Erfan Rahmani, Mehrab Pourmadadi, Sohrab Ali Ghorbanian, Fatemeh Yazdian, Hamid Rashedi, Mona Navaee
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引用次数: 46

Abstract

Despite its widespread usage as a chemotherapy drug in cancer treatment, doxorubicin (DOX) has limitations such as short in vivo circulation time, low solubility, and poor permeability. In this regard, a pH-responsive chitosan (CS)- montmorillonite (MMT)- nitrogen-doped carbon quantum dots (NCQDs) nanocomposite was first developed, loaded with DOX, and then incorporated into a double emulsion to further develop the sustained release. The incorporated NCQDs into the CS-MMT hydrogel exhibited enhanced loading and entrapment efficiencies. The presence of NCQDs nanoparticles in the CS-MMT hydrogel also resulted in an extended pH-responsive release of DOX over a period of 96 h compared to that of CS-MMT-DOX nanocarriers at pH 5.4. Based on the Korsmeyer-Peppas model, there was a controlled DOX release at pH 5.4, while no diffusion was observed at pH 7.4, indicating fewer side effects. MTT assay showed that the cytotoxicity of DOX-loaded CS-MMT-NCQDs hydrogel nanocomposite was significantly higher than those of free DOX (p < 0.001) and CS-MMT-NCQDs (p < 0.001) on MCF-7 cells. Flow cytometry results demonstrated that a higher apoptosis induction achieved after incorporating NCQDs nanoparticles into CS-MMT-DOX nanocarrier. These findings suggest that the DOX-loaded nanocomposite is a promising candidate for the targeted treatment of cancer cells.

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ph响应壳聚糖-蒙脱石-氮掺杂碳量子点纳米载体的制备以减弱阿霉素在癌症治疗中的局限性
多柔比星(DOX)作为化疗药物广泛应用于癌症治疗,但存在体内循环时间短、溶解度低、渗透性差等局限性。为此,首先制备了ph响应型壳聚糖(CS)-蒙脱土(MMT)-氮掺杂碳量子点(NCQDs)纳米复合材料,负载DOX,然后将其掺入双乳液中进一步发展缓释。将NCQDs加入到CS-MMT水凝胶中,显示出更高的负载和捕集效率。与CS-MMT-DOX纳米载体相比,CS-MMT水凝胶中NCQDs纳米颗粒的存在也导致DOX在pH值为5.4时的pH响应释放时间延长了96小时。根据Korsmeyer-Peppas模型,pH为5.4时DOX释放受控制,而pH为7.4时未观察到扩散,表明副作用较少。MTT实验表明,负载DOX的CS-MMT-NCQDs水凝胶纳米复合材料的细胞毒性显著高于游离DOX (p <0.001)和CS-MMT-NCQDs (p <0.001)对MCF-7细胞的影响。流式细胞术结果表明,将NCQDs纳米颗粒加入CS-MMT-DOX纳米载体后,细胞凋亡诱导率更高。这些发现表明,负载dox的纳米复合材料是一种有希望的靶向治疗癌细胞的候选材料。
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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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Issue Information Cover Picture: Engineering in Life Sciences 11'24 Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency Issue Information Cover Picture: Engineering in Life Sciences 10'24
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