In vivo imaging study of angiogenesis in a channelized porous scaffold.

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS Molecular Imaging Pub Date : 2015-01-01 DOI:10.2310/7290.2015.00011
Margherita Tamplenizza, Alessandro Tocchio, Irini Gerges, Federico Martello, Cristina Martelli, Luisa Ottobrini, Giovanni Lucignani, Paolo Milani, Cristina Lenardi
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引用次数: 4

Abstract

The main scientific issue hindering the development of tissue engineering technologies is the lack of proper vascularization. Among the various approaches developed for boosting vascularization, scaffold design has attracted increasing interest over the last few years. The aim of this article is to illustrate a scaffold design strategy for enhancing vascularization based on sacrificial microfabrication of embedded microchannels. This approach was combined with an innovative poly(ether urethane urea) (PEUtU) porous scaffold to provide an alternative graft substitute material for the treatment of tissue defects. Fluorescent and chemiluminescent imaging combined with computed tomography were used to study the behavior of the scaffold composition within living subjects by analyzing angiogenesis and inflammation processes and observing the variation in x-ray absorption, respectively. For this purpose, an IntegriSense 680 probe was used in vivo for the localization and quantification of integrin αvβ3, due to its critical involvement in angiogenesis, and a XenoLight RediJect Inflammation Probe for the study of the decline in inflammation progression during healing. Overall, the collected data suggest the advantages of embedding a synthetic vascular network into a PEUtU porous matrix to enhance in vivo tissue integration, maturation, and regeneration. Moreover, our imaging approach proved to be an efficient and versatile tool for scaffold in vivo testing.

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通道化多孔支架血管生成的体内成像研究。
阻碍组织工程技术发展的主要科学问题是缺乏适当的血管化。在促进血管化的各种方法中,支架设计在过去几年中引起了越来越多的兴趣。本文的目的是阐述一种基于嵌入式微通道牺牲微加工的支架设计策略,以增强血管化。该方法与创新的聚醚脲(PEUtU)多孔支架相结合,为治疗组织缺陷提供了一种替代的移植物替代材料。荧光和化学发光成像结合计算机断层扫描,分别通过分析血管生成和炎症过程以及观察x射线吸收的变化来研究支架成分在活体体内的行为。为此,我们在体内使用IntegriSense 680探针来定位和定量整合素αvβ3,因为它在血管生成中起关键作用;使用XenoLight RediJect炎症探针来研究愈合过程中炎症进展的下降。总的来说,收集到的数据表明,将合成血管网络嵌入PEUtU多孔基质中,可以增强体内组织的整合、成熟和再生。此外,我们的成像方法被证明是一种高效和通用的支架体内测试工具。
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来源期刊
Molecular Imaging
Molecular Imaging Biochemistry, Genetics and Molecular Biology-Biotechnology
自引率
3.60%
发文量
21
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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