Phage L5 integrating vectors are present within the Mycobacterial Cell in an equilibrium between integrated and excised states.

Cancer therapy Pub Date : 2009-01-01
Beatrice Saviola
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Abstract

Integrating mycobacterial plasmids containing the phage L5 attachment site (attP) are able to insert into the mycobacterial chromosome attB site. Plasmids containing the attP site and chromosome containing the attB site are present in equilibrium between the inserted and the excised states in the presence of the phage L5 integrase.

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噬菌体L5整合载体存在于分枝杆菌细胞内,处于整合和切除状态之间的平衡状态。
整合含有噬菌体L5附着位点(attP)的分枝杆菌质粒能够插入到分枝杆菌染色体attB位点。在噬菌体L5整合酶存在的情况下,含有attP位点的质粒和含有attB位点的染色体在插入和切除状态之间处于平衡状态。
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