[Determination of Azospirillum Brasilense Cells With Bacteriophages via Electrooptical Analysis of Microbial Suspensions].

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2015-05-01 DOI:10.7868/s0555109915030083

O I Gulii, O A Karavayeva, S A Pavlii, O I Sokolov, V D Bunin, O V Ignatov

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Abstract

The dependence-of changes in the electrooptical properties of Azospirillum brasilense cell suspension Sp7 during interaction with bacteriophage ΦAb-Sp7 on the number and time of interactions was studied. Incubation of cells with bacteriophage significantly changed the electrooptical signal within one minute. The selective effect of bacteriophage ΦAb on 18 strains of bacteria of the genus Azospirillum was studied: A. amazonense Ami4, A. brasilense Sp7, Cd, Sp107, Sp245, Jm6B2, Brl4, KR77, S17, S27, SR55, SR75, A. halopraeferans Au4, A. irakense KBC1, K A3, A. lipoferum Sp59b, SR65 and RG20a. We determined the limit of reliable determination of microbial cells infected with bacteriophage: - 10(4) cells/mL. The presence of foreign cell cultures of E. coli B-878 and E. coli XL-1 did not complicate the detection of A brasilense Sp7 cells with the use of bacteriophage ΦAb-Sp7. The results demonstrated that bacteriophage (ΦAb-Sp7 can be used for the detection of Azospirillum microbial cells via t electrooptical analysis of cell suspensions.

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[微生物悬浮液电光分析噬菌体测定巴西氮螺旋菌细胞]。

研究了巴西氮螺旋藻(Azospirillum brasilense)细胞悬浮液Sp7与噬菌体ΦAb-Sp7相互作用过程中电光性质的变化与相互作用次数和时间的关系。与噬菌体孵育的细胞在一分钟内显著改变了电光信号。研究了噬菌体ΦAb对18株氮螺旋藻属细菌的选择性作用:A. amazonense Ami4、A. brasilense Sp7、Cd、Sp107、Sp245、Jm6B2、Brl4、KR77、S17、S27、SR55、SR75、A. halopraeferans Au4、A. irakense KBC1、K A3、A. lipoferum Sp59b、SR65和RG20a。我们确定了被噬菌体感染的微生物细胞的可靠检测限:- 10(4)个细胞/mL。大肠杆菌B-878和大肠杆菌XL-1的外源细胞培养物的存在并未使使用噬菌体ΦAb-Sp7检测巴西螺孢子虫Sp7细胞变得复杂。结果表明，噬菌体(ΦAb-Sp7)可以通过对细胞悬浮液的光电分析，用于偶氮螺旋藻微生物细胞的检测。

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Prikladnaia biokhimiia i mikrobiologiia

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