[Analysis of Chlorophyll Fluorescence Transient by Spectral Multi-exponential Approximation].

Biofizika Pub Date : 2015-05-01
T Yu Plyusnina, S S Klruschev, G Yu Riznichenko, A B Rubin
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Abstract

The method for analysis of chlorophyll fluorescence transient using approximation of measured signal by multi-exponential series is described. Visualization of partial sums of this series allows us to find amplitudes and characteristic times of individual phases of fluorescence induction curve. This method gives more rigid criteria of phase identification instead of semi-empirical approach currently used. Applied to Chlamidomonas reinhardtii sulfur deprivation case, it shows efficiency in finding visually undistinguishable phases of fluorescence transient for early detection of stress.

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[光谱多指数近似分析叶绿素荧光瞬态]。
介绍了用多指数级数逼近测量信号分析叶绿素荧光瞬态的方法。该序列部分和的可视化使我们能够找到荧光诱导曲线各相的振幅和特征时间。该方法代替了目前使用的半经验方法,给出了更严格的相识别标准。将该方法应用于莱茵衣单胞菌的硫剥夺案例中,发现了视觉上难以区分的荧光瞬态相,可用于胁迫的早期检测。
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