[Investigation of Free Radical Processes in Substrates and Biological Samples by Means of induced Chemiluminescence].

Abstract

The possibility of applying induced chemiluminescence was investigated to evaluate oxidative capability of the substrate under the action of hydroxyl radicals and estimate the intensity of free radical processes in biological samples based on the analysis of the organic hydroperoxyde response in a probe. For this purpose the chemiluminescence light sum was measured in 3 steps: when Fe2+ was introduced into in a sample; in course of Fenton reaction (introducing Fe2+ and H202) and when Fe2+ was introduced into the sample after Fenton reaction. Light sum was measured depending on concentration (dilution) of the sample. It was shown that the light sum reaches the maximum value at certain dilution of the substrate studied. The maximum chemiluminescence's position is determined by concentration of RH fragments being oxidized, but the chemoluminescence light sum value is determined partially by the inhibitor [InH]/[RH] and organic hydroperoxides [ROOH]/[RH] found in the sample.