[Biodegradation of Cellulose-Containing Substrates by Micromycetes Followed by Bioconversion into Biogas].

L I Prokudina, A A Osmolovskii, M A Egorova, D V Malakhova, A I Netrusov, E A Tsavkelova
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Abstract

The ability of micromycetes Trichoderma viride and Aspergillus terreus to decompose the cellulose-containing substrates was studied. Office paper and cardboard, as well as a paper mixture, were found to be the most hydrolyzable. The cellulolytic activity of T. viride was 2-3 times higher than that of A. terreus; the highest values of 0.80 and 0.73 U/mLwere obtained from office paper and the paper mixture, respectively. The micromycete cultivation conditions (composition of culture medium, sucrose cosubstrate addition, seeding method) and the conditions of the fungus biomass treatment for its subsequent bioconversion into biogas by anaerobic microbial communities were optimized. It was shown that pretreatment improves the efficiency of biogas production from lignocellulosic materials under seeding with microbial community of bovine animal manure. After pretreatment of the Jerusalem artichoke phytomass (stems and leaves) and its subsequent bioconversion into biogas by methanogenic community, the biogas yield was increased by 1.5 times.

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[微真菌对含纤维素底物的生物降解及转化为沼气]。
研究了绿木霉和土曲霉对含纤维素底物的分解能力。研究发现,办公用纸和硬纸板以及纸张混合物最易水解。绿毛霉的纤维素分解活性比土霉高2 ~ 3倍;办公用纸和混合纸的浓度最高,分别为0.80和0.73 U/ ml。优化了微菌培养条件(培养基组成、蔗糖共底物添加、播种方式)和厌氧微生物群落将真菌生物量转化为沼气的处理条件。结果表明,在牛粪微生物群落播种的情况下,预处理提高了木质纤维素原料产沼气的效率。菊芋植物(茎叶)经预处理后由产甲烷菌群转化为沼气,其产气量提高1.5倍。
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