Green spectrofluorimetric determination of alendronate sodium using nano switchable probe; Water-soluble carbon dots synthesized from a natural source

Abstract

Kidney beans were effectively pyrolyzed to produce N-carbon dots (N-CDs) with bright blue fluorescence. The mono-step pyrolytic synthesis was easy to carry out and resulted in water soluble green N-CDs with higher quantum yield 40.61%. Firstly, the fluorescence of N-CDs was turned off by Ferric ions (Fe³⁺); Alendronate sodium can recover the fluorescence of N-CDs-Fe³⁺. N-CDs worked as a highly specific detection probe to determine alendronate sodium in pure and pharmaceutical dosage form without interference from co-formulated cholecalciferol. The sensing system showed a good linear relationship to determine alendronate sodium within concentration (1.00−25.00μg/mL), with detection and quantification limits of 0.3μg/mL and 1.04μg/mL, respectively.