Talanta Open最新文献

{"title":"Rapid RP-HPLC method for simultaneous quantification of fenoterol hydrobromide and ipratropium bromide in breathable and respirable fraction from nebulizers","authors":"C. Chilka,&nbsp;J. Dhalani","doi":"10.1016/j.talo.2026.100621","DOIUrl":"10.1016/j.talo.2026.100621","url":null,"abstract":"<div><div>The present research study was designed to evaluate the breathable fraction as well as the aerodynamic characteristics of the fenoterol hydrobromide (FEN) and ipratropium bromide (IPR) using a Next Generator Impactor (NGI) and sensitive reverse phase high pressure liquid chromatography. The aerodynamic particle size distribution (APSD) is measured using the NGI, a cascade impactor instrument. The APSD mimics particle deposition in various respiratory tracts after inhalation depending on the aerodynamic diameter of the medication. The samples were analysed using X-bridge C18 (50 mm x 4.6 mm), 3.5 µm column with mobile phase consisting of 80:20 % v/v mixture of heptane-1-sulfonic acid sodium salt and acetonitrile. The analytical technique was validated and the respirable fraction of the nebulized dose was calculated as an extension of the method application. The average respirable mass (≤ 5μm in particle size, penetrate deep into the human lungs) for Fenoterol hydrobromide and Ipratropium bromide were observed 226.596 µg and 112.357 µg, respectively. The average breathable fraction (the portion of aerosol particles inhaled by human) for Fenoterol hydrobromide and Ipratropium bromide were observed 294.901 µg and 146.472 µg, respectively. The analytical technique introduced to evaluate breathable mass and respirable mass of Fenoterol hydrobromide and Ipratropium bromide nebulizer solution produces a reliable result with high sensitivity and reproducibility. The shorter runtime and cost effectiveness enhance the method’s suitability to its routine use in quality control. The presented work enhances the applicability for routine aerodynamic assessment with compliance of regulatory and pharmacopeial guidelines in pharmaceutical industry.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100621"},"PeriodicalIF":3.7,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecofriendly dual-emission cerium, nitrogen, and sulfur co-doped carbon dots as a ratiometric probe for spironolactone: An antioxidant-based approach","authors":"Hamsa B. Hassan ,&nbsp;Mokhtar M. Mabrouk ,&nbsp;Fotouh R. Mansour ,&nbsp;Amira H. Kamal ,&nbsp;Aya A. Abdella","doi":"10.1016/j.talo.2026.100619","DOIUrl":"10.1016/j.talo.2026.100619","url":null,"abstract":"<div><div>Ratiometric probes are analytical tools that utilize a single probe with two or more emission signals to measure analyte concentrations, effectively correcting for measurement variations. Although several methods have been previously employed for spironolactone (SPR) determination, these methods often require expensive instrumentation, lengthy extraction or derivatization steps, and generate considerable chemical waste. In this study, a facile, rapid, and environmentally friendly ratiometric spectrofluorometric method for determining SPR is presented. Dual emission carbon dots co-doped with cerium, nitrogen, and sulfur (Ce-NSCDs) were formed through one-pot microwave-assisted pyrolysis, utilizing citric acid, urea, and ammonium cerium (IV) sulfate as precursors in just 60 seconds, for the first time. The Ce-NSCDs displayed dual emissions at 350 nm and 430 nm when excited at 320 nm. Notably, SPR enhanced the emission of Ce-NSCDs at 350 nm, while the emission at 430 nm remained unaffected. Transmission electron microscopy images indicated that SPR interacted with the surface of Ce-NSCDs, forming a shell. X-ray photoelectron spectroscopy investigations suggested a Ce(IV)/Ce(III) redox mechanism, where the intrinsic antioxidant activity of SPR enabled the modulation of the Ce(IV)/Ce(III) equilibrium, leading to enhanced emission. Consequently, a ratiometric sensing probe was developed based on the F₃₅₀/F₄₃₀ emission ratio. The determination of SPR demonstrated excellent linearity (R² = 0.996) over a concentration range of 40 - 200 μg/mL, with a limit of detection of 13.08 μg/mL, and exhibited good accuracy and precision (% recovery of 100 ± 2), and selectivity with excellent reproducibility (%RSD &lt; 2%), confirming its precision and reliability. This innovative sensing strategy was successfully applied for the determination of SPR in pharmaceutical formulations, yielding satisfactory recovery results that demonstrate the method’s practical applicability, and was shown to be environmentally benign.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100619"},"PeriodicalIF":3.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasmall CuFeMn nanozymes with multienzyme activity under neutral conditions: A smartphone-based colorimetric visualization of multiple biomarkers","authors":"Haiyi Xiong ,&nbsp;Liang Ye ,&nbsp;Na Ni ,&nbsp;Yan Zhang ,&nbsp;Jingkun Miao ,&nbsp;Jun Chen","doi":"10.1016/j.talo.2026.100620","DOIUrl":"10.1016/j.talo.2026.100620","url":null,"abstract":"<div><div>This study presents a novel nanozyme design with the development of ultrasmall trimetallic CuFeMn nanozymes (CF4M), which uniquely demonstrate dual peroxidase (POD)-like and oxidase (OXD)-like enzymatic activities under neutral pH conditions. By circumventing the limitation of conventional nanozymes that require acidic environments for optimal function, CF4M enables biosensing workflows without the need for buffer adjustments, significantly enhancing practical applicability. The CF4M utilizes its multivalent properties of Cu, Fe and Mn to ensure POD-like and OXD-like enzyme activity in neutral environments, addressing a major challenge in the field of biosensing. A versatile colorimetric platform utilizing CF4M achieves detection of multiple biomarkers, including glutathione (GSH), glucose (Glu), and glucose-6-phosphate dehydrogenase (G6PD) through cascading enzymatic reactions and TMB chromogenic responses. Integrated with smartphone-based imaging technology mediated by gold nanorods, the system facilitates user-friendly and visual quantification of multiple biomarkers. The CF4M-based biosensing platform establish a new paradigm for neutral-pH-compatible biosensors, offering broad utility in detect clinical biomarkers.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100620"},"PeriodicalIF":3.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics and Nanoplastics in human tissues: Systematic review of evidence, analytical protocols, and methodological challenges","authors":"Simone Ottavio Zielli ,&nbsp;Jennifer Paola Pascali ,&nbsp;Antonio Mazzotti ,&nbsp;Paolo Fais ,&nbsp;Milena Fini ,&nbsp;Cesare Faldini ,&nbsp;Susi Pelotti","doi":"10.1016/j.talo.2026.100615","DOIUrl":"10.1016/j.talo.2026.100615","url":null,"abstract":"<div><div>Microplastics and NanoPlastics (MNP) have emerged as ubiquitous environmental contaminants with potential implications for human health. This systematic review synthesizes current evidence on the occurrence of MNP in human solid tissues and critically evaluates the analytical protocols employed for their detection and quantification. A comprehensive literature search conducted in September 2025 across MEDLINE, EMBASE, and the Cochrane Library, in accordance with PRISMA guidelines, identified 26 eligible studies encompassing 564 human samples from diverse biological matrices, including placenta, lung, liver, blood, and bone.</div><div>Polyethylene, polypropylene, and polyvinyl chloride were the most frequently detected polymers, while particle sizes predominantly ranged between 20 and 100 µm. Analytical techniques varied substantially across studies, with Raman and Fourier-transform infrared (FTIR) spectroscopy representing the most widely applied methods, often complemented by microscopy or pyrolysis–GC/MS for polymer confirmation. Reported MNPs abundances ranged from less than one to several thousand particles per gram of tissue, reflecting the lack of standardized procedures for extraction, quantification, and contamination control.</div><div>Recent investigations have increasingly implemented plastic-free workflows and procedural blanks, leading to improved reliability and reduced overestimation of MNP burden. Nevertheless, persistent methodological heterogeneity continues to hinder cross-study comparability and the establishment of true human tissue loads. Preliminary correlations between MNP accumulation and clinical or pathological parameters have been observed, but causal links remain unconfirmed.</div><div>This review highlights the urgent need for internationally harmonized protocols, rigorous contamination prevention, and standardized reporting to ensure reliable biomonitoring and clarify the potential health implications of microplastic exposure in humans.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100615"},"PeriodicalIF":3.7,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Off - on fluorescent probe using sulfur-doped graphitic carbon nitride nanosheets for detection of copper ion and cysteine","authors":"Obwodo Omod Obang ,&nbsp;Alemensh Bekele ,&nbsp;Fuad Abduro Bushira ,&nbsp;Alemayehu Yifru ,&nbsp;Tadesse Haile Fereja ,&nbsp;Shimeles Addisu Kitte","doi":"10.1016/j.talo.2026.100618","DOIUrl":"10.1016/j.talo.2026.100618","url":null,"abstract":"<div><div>A sensitive off-on fluorescent probe was demonstrated using sulfur-doped graphitic carbon nitride nanosheets (S-g-C₃N₄ NSs) for the sequential determination of copper ions (Cu²⁺) and cysteine (Cys). The S-g-C₃N₄ NSs, synthesized via simple thermal polymerization method, exhibited strong fluorescence, which was selectively quenched by Cu²⁺ via a photoinduced electron transfer (PET) mechanism. The resulting S-g-C₃N₄/Cu²⁺complex then served as an effective platform for sensing Cys, which restored the fluorescence by competitively binding Cu²⁺. The probe demonstrated excellent sensitivity with low limit of detection (LOD) of 0.033 µM for Cu²⁺and 19 nM for Cys, high selectivity over potential interferents, and good stability. The method was successfully applied for the determination of Cu²⁺in tap water and Cys in multivitamin tablets, achieving satisfactory recoveries, which confirms its potential for practical environmental and biological monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100618"},"PeriodicalIF":3.7,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthracene boronic acid methacrylate-based fluorescence activation assay for determination of glycated proteins in biological samples","authors":"Jaroslava Bezdekova ,&nbsp;Kristyna Pavelicova ,&nbsp;Dattatry Shivajirao Bhosale ,&nbsp;Silvie Kozakova ,&nbsp;Lenka Pavlikova ,&nbsp;Jihao Yu ,&nbsp;Jan Bartacek ,&nbsp;Jan Svoboda ,&nbsp;Milos Sedlak ,&nbsp;Andrew D. Miller ,&nbsp;Marketa Vaculovicova","doi":"10.1016/j.talo.2026.100617","DOIUrl":"10.1016/j.talo.2026.100617","url":null,"abstract":"<div><div>There is a current unmet medical need for the creation and development of point-of-care diagnostic tests/devices that can determine if a given individual is diabetic, pre-diabetic or normal. In pursuit of this objective, we report here on the successful evaluation of a bespoke anthracene boronic acid methacrylate (ABAM) in a rapid and simple fluorescence assay for the detection of glycated proteins known as disease biomarkers in human plasma samples. In so doing, we determine that increases in ABAM fluorescence intensity, as a function of glycated human serum albumin (gHSA) levels, in 50-fold diluted plasma samples, are sufficiently well resolved to suggest that ABAM could form the basis of a potential point-of-care diagnostic device to discriminate between blood plasma samples taken from individuals who are diabetic, pre-diabetic or normal. Indeed, our ABAM-based assay is shown to discriminate clearly in 50-fold diluted plasma samples between increases in gHSA plasma concentrations (from 5 mg L⁻¹ to 65 mg L⁻¹) above the normal base line, reaching final concentrations indicative of diabetes. In comparison to the nearest competitor, the fructosamine detection assay, our ABAM-based assay is twelve times faster, more than six times less expensive, requires ten times lower sample volumes, is both gHSA selective and specific, and is much less prone to interference from molecular interferents. Therefore, we would suggest that our ABAM-based assay has real future potential utility in diabetes disease management.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100617"},"PeriodicalIF":3.7,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an analytical method for simultaneous determination of 38 bile acids and its application in studying the mechanism of WuZhi Pian against rifampicin-induced liver injury","authors":"Caiping Zhang ,&nbsp;Jingyu Li ,&nbsp;Jianan Zheng ,&nbsp;Yiming He ,&nbsp;Yongxv Chen ,&nbsp;Fangli Liu ,&nbsp;Zhongqiu Liu ,&nbsp;Zhuoru He","doi":"10.1016/j.talo.2026.100607","DOIUrl":"10.1016/j.talo.2026.100607","url":null,"abstract":"<div><div>Bile acids (BAs) are crucial signaling molecules involved in liver metabolism regulation. The development of quantitative detection methods for BAs is essential for understanding the molecular mechanisms of liver-related diseases. In this study, we optimized an analytical method to quantify 38 BAs in serum using ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS). We evaluated and optimized various sample preparation methods, chromatographic columns, mobile phase compositions, and mass spectrometry conditions. After method validation, BA profiles were analyzed in mice with rifampicin (RIP)-induced liver injury, with or without WuZhi Pian treatment. According to the results, the optimal serum preparation involved a 1% ammonia-methanol solution at a 1:3 sample-to-solvent ratio, vortex-mixing for 5 min, and incubating for 60 min at 4°C. All 38 BAs achieved optimal separation on an ACQUITY UPLC® HSS T3 column (2.1 × 100 mm, 1.8 μm) with a 20-min run time. The best peak shapes and responses were obtained using mobile phases consisted of 4.3 mM ammonia with 0.1% acetic acid in water and acetonitrile. This method demonstrated high specificity, linearity (&gt; 0.9991), and a lower limit of quantification (LLOQ) ranging from 0.0054 to 2.4414 nM across different BAs, with good repeatability (RSD% &lt; 15.35%) and reproducibility (RSD% &lt; 19.68%). Average recoveries for all BAs ranged from 70 to 122%. Using this method, 13 BAs were found to be altered in RIP-induced liver injury, of which nine BAs were modulated by WuZhi Pian treatment. Therefore, the optimized UPLC-QQQ-MS/MS method is a powerful tool for monitoring BA metabolism-related diseases.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100607"},"PeriodicalIF":3.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perovskite nanocrystals as fluorescent sensors for Fe³⁺: Mechanistic insights and emerging trends","authors":"Mohamed Abu Shuheil ,&nbsp;G. PadmaPriya ,&nbsp;Subhashree Ray ,&nbsp;Talal Aziz Qassem ,&nbsp;Gunjan Garg ,&nbsp;Renu Sharma ,&nbsp;Bekzod Madaminov ,&nbsp;Sabokhat Sadikova ,&nbsp;Shayan Mahmoodi","doi":"10.1016/j.talo.2026.100616","DOIUrl":"10.1016/j.talo.2026.100616","url":null,"abstract":"<div><div>Perovskite nanocrystals (PNCs) have emerged as powerful fluorescent platforms for the selective and sensitive detection of Fe³⁺ ions due to their defect-tolerant electronic structure, high photoluminescence efficiency, and tunable optoelectronic properties. This review provides mechanistic insights into the diverse quenching pathways governing Fe³⁺ sensing, including photoinduced electron transfer, inner filter effects, cation exchange, aggregation dynamics, magnetic polaron formation, and oxidative surface processes. By correlating structural characteristics with optical responses, we highlight how composition engineering, surface chemistry, and dimensionality influence sensing performance across lead-based and lead-free systems. Emerging trends such as ratiometric emission, core–shell stabilization, hybrid organic–inorganic interfaces, and dopant-mediated enhancements are discussed as promising strategies toward improved accuracy, environmental stability, and biocompatibility. Recent advances in film-based and solid-state perovskite sensors further underscore their potential for integration into portable, low-cost detection platforms. To the best of our knowledge, this work represents the first review article specifically focused on Fe³⁺ ion sensing using PNCs, offering a unified perspective on mechanistic foundations and future directions. Overall, this work consolidates current progress while identifying challenges and opportunities for developing next-generation perovskite fluorescent sensors capable of reliable Fe³⁺ monitoring in environmental, biological, and industrial settings.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100616"},"PeriodicalIF":3.7,"publicationDate":"2026-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AIE-active ferrocene conjugated imidazole fluorescent chromophores for “turn on/off” detection of hydrogen peroxide in mixed aqueous media and bio-imaging applications","authors":"Selvam Prabu,&nbsp;Nallasamy Palanisami","doi":"10.1016/j.talo.2026.100614","DOIUrl":"10.1016/j.talo.2026.100614","url":null,"abstract":"<div><div>We have synthesized ferrocene-appended Y-shaped methoxyphenyl-substituted imidazole derivatives 2-ferrocenyl-4,5-bis(4-methoxyphenyl)-1H-imidazole (<strong>1</strong>) and 2-ferrocenyl-4,5-bis((E)-4-methoxystyryl)-1H-imidazole (<strong>2</strong>), and characterized them using analytical and spectroscopic techniques (¹H, ¹³C NMR, FT-IR, and HR-Mass). Optical properties, including absorption, emission, quantum yield, and aggregation-induced emission (AIE), were demonstrated. The emission, ranging from weak to enhanced, was achieved through the AIE process in CH₃CN/H₂O mixtures by restricting intramolecular rotation (RIR). In the aggregated state, the quantum efficiency of chromophores <strong>1</strong> and <strong>2</strong> increases threefold and twofold, respectively, compared to the pure CH₃CN solution. Utilizing the AIE state, these chromophores were further employed to detect hydrogen peroxide (H₂O₂) in a mixed aqueous medium <em>via</em> a fluorescence <em>turn-on/off</em> approach. This was conducted with water fractions (<em>f_w</em>) of 80 % for chromophore <strong>1</strong> and 70 % for chromophore <strong>2</strong>, revealing a highly specific and sensitive fluorescence quenching response to H₂O₂ in the aqueous mixture. The response is rapid within a linear range of 0–110 µM and 0–80 µM, with detection thresholds of 24.7 nM for chromophore <strong>1</strong> and 30.9 nM for chromophore <strong>2</strong>, respectively. The limited detection capability is attributed to the absence of an alkene group in the chromophore, leading to low sensitivity due to the chromophore's high molecular rigidity, which impedes molecular motion. Moreover, chromophores <strong>1</strong> and <strong>2</strong> are non-cytotoxic (cell viability above 80 %), enabling them to detect intracellular H₂O₂ in human cervical carcinoma (HeLa) cells through fluorescence bio-imaging. Notably, the significant bright green fluorescence is quenched upon the addition of H₂O₂. Additionally, DFT/B3LYP calculations were used to explore the HOMO and LUMO energy levels and charge distribution of the optimized structure. The calculated HOMO and LUMO energies were compared with the experimentally obtained redox potential values.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100614"},"PeriodicalIF":3.7,"publicationDate":"2026-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new paradigm for the discrimination of Polygonatum geographical authenticity based on the coupling of stable isotope and secondary metabolites","authors":"Xiang Zhu ,&nbsp;Qian Li ,&nbsp;Yumeng Xia ,&nbsp;Qianyu Yao ,&nbsp;Zengcai Liang ,&nbsp;Yanan Wu ,&nbsp;Yan Zhao ,&nbsp;Weijun Guan ,&nbsp;Taofeng Lu","doi":"10.1016/j.talo.2026.100610","DOIUrl":"10.1016/j.talo.2026.100610","url":null,"abstract":"<div><h3>Background</h3><div>The genus <em>Polygonatum,</em> collectively known as Polygonatum or Solomon’s Seal, contains a number of species that have edible tuber-like stems. Technology to trace the origin of harvested plant materials is crucial. Traditional stable isotope tracing utilizes C, H, O, N and even other mineral elements for cluster analysis. This method currently has some shortcomings; if the sample size is small, the resolution will decrease. Additionally, if more stable isotope contents are measured, the costs will significantly increase. <strong>It is evident that it is necessary to enhance the accuracy of origin discrimination analysis without increasing the sample size or incurring excessive costs.</strong> (101)</div></div><div><h3>Results</h3><div>Our study selected five substances: <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and polygona-polysaccharose in Polygonatum tuber-like stem to distinguish the origin of samples from six cities in southern China. The accuracy of both the original classification and cross-validation accuracy reached 100 %. The use of only four stable substances: <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H and <em>δ</em>¹⁸O has reduced the accuracy of original classification and cross-validation to 84.4 % and 75.6 %. In addition, we studied the correlation between <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and the content of polygona-polysaccharose in Polygonatum samples. There is a linear positive correlation between <em>δ</em>²H and <em>δ</em>¹⁸O from six cities in south China. The <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and the content of polygona-polysaccharose and seven environmental parameters were also tested. We found a significant positive correlation between <em>δ</em>¹⁸O vs. latitude, and a significant positive correlation between <em>δ</em>²H vs. longitude and <em>δ</em>²H vs. annual evaporation. (138)</div></div><div><h3>Significance</h3><div>Our study represents the first origin determination results of the stable isotope of Polygonatum. The accuracy and precision of origin identification using stable isotopes was enhanced by adding the content of polygona-polysaccharose even with a limited sample size. The linear relationship between the proportion of <em>δ</em>²H and <em>δ</em>¹⁸O in the tuber-like stems of Polygonatum could help us distinguish Polygonatum from other tuber foods. (63)</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100610"},"PeriodicalIF":3.7,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}