Talanta Open最新文献

{"title":"Nanoparticle-based lateral flow immunoassays for detecting marine biotoxins in seafood: a systematic review of trends in development over two decades","authors":"Perveen Akhtar ,&nbsp;Gayathree Thenuwara ,&nbsp;Hugh J. Byrne ,&nbsp;Furong Tian ,&nbsp;Bilal Javed","doi":"10.1016/j.talo.2025.100604","DOIUrl":"10.1016/j.talo.2025.100604","url":null,"abstract":"<div><div>Marine and freshwater biotoxins threaten public health and seafood safety due to their tendency to accumulate in fish and shellfish. This systematic literature review summarises findings from 51 peer-reviewed studies published between 2004 and 2025 that focus on nanoparticle-enhanced lateral flow immunoassays (LFIAs) for toxin detection. Gold nanoparticles (AuNPs) were by far the most used label, employed in ∼75 % of studies, which consistently delivered the lowest limits of detection (LODs) as low as 0.03 ng/ml for okadaic acid detection. Despite increasing concerns over the co-occurrence of multiple toxins, ∼88 % of the studies were based on single-analyte detection, only ∼12 % employing multiplexed approaches. Statistical analyses of the studies, namely Kruskal–Wallis, Dunn’s post hoc, correlation, and regression analysis, revealed meaningful trends. LODs have significantly improved over time, a marked reduction being observed after 2016. Kruskal–Wallis and Dunn’s post hoc tests showed significant differences across variables such as nanoparticle size, assay duration, and toxin recovery. Spearman correlation results identified a moderately significant negative correlation of LOD with publication year (ρ = –0.458, <em>p</em> &lt; 0.001), and a weaker significant correlation of LOD with assay time (ρ = –0.301, <em>p</em> = 0.018), while linear regression analysis identified publication year (R² = 0.2441, <em>p</em> &lt; 0.0001) and nanoparticle size (R² = 0.1072, <em>p</em> = 0.0264) as significant predictors of assay sensitivity. Overall, this review underscores the central role of nanoparticle engineering, simplified assay workflows, and optimized design strategies in improving LFIA performance. The findings provide a valuable roadmap for advancing the development of portable, sensitive, and scalable biosensing platforms for marine toxin monitoring and seafood safety surveillance.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100604"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecofriendly dual-emission cerium, nitrogen, and sulfur co-doped carbon dots as a ratiometric probe for spironolactone: An antioxidant-based approach","authors":"Hamsa B. Hassan ,&nbsp;Mokhtar M. Mabrouk ,&nbsp;Fotouh R. Mansour ,&nbsp;Amira H. Kamal ,&nbsp;Aya A. Abdella","doi":"10.1016/j.talo.2026.100619","DOIUrl":"10.1016/j.talo.2026.100619","url":null,"abstract":"<div><div>Ratiometric probes are analytical tools that utilize a single probe with two or more emission signals to measure analyte concentrations, effectively correcting for measurement variations. Although several methods have been previously employed for spironolactone (SPR) determination, these methods often require expensive instrumentation, lengthy extraction or derivatization steps, and generate considerable chemical waste. In this study, a facile, rapid, and environmentally friendly ratiometric spectrofluorometric method for determining SPR is presented. Dual emission carbon dots co-doped with cerium, nitrogen, and sulfur (Ce-NSCDs) were formed through one-pot microwave-assisted pyrolysis, utilizing citric acid, urea, and ammonium cerium (IV) sulfate as precursors in just 60 seconds, for the first time. The Ce-NSCDs displayed dual emissions at 350 nm and 430 nm when excited at 320 nm. Notably, SPR enhanced the emission of Ce-NSCDs at 350 nm, while the emission at 430 nm remained unaffected. Transmission electron microscopy images indicated that SPR interacted with the surface of Ce-NSCDs, forming a shell. X-ray photoelectron spectroscopy investigations suggested a Ce(IV)/Ce(III) redox mechanism, where the intrinsic antioxidant activity of SPR enabled the modulation of the Ce(IV)/Ce(III) equilibrium, leading to enhanced emission. Consequently, a ratiometric sensing probe was developed based on the F₃₅₀/F₄₃₀ emission ratio. The determination of SPR demonstrated excellent linearity (R² = 0.996) over a concentration range of 40 - 200 μg/mL, with a limit of detection of 13.08 μg/mL, and exhibited good accuracy and precision (% recovery of 100 ± 2), and selectivity with excellent reproducibility (%RSD &lt; 2%), confirming its precision and reliability. This innovative sensing strategy was successfully applied for the determination of SPR in pharmaceutical formulations, yielding satisfactory recovery results that demonstrate the method’s practical applicability, and was shown to be environmentally benign.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100619"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal-free perylene-based polyimide frameworks for oxygen reduction-enhanced electrochemiluminescence biosensing","authors":"Qiao Chen ,&nbsp;Xiaoran Zhang ,&nbsp;Xinyue Zhou ,&nbsp;Peng Huang ,&nbsp;Daming Feng ,&nbsp;Xu Xu ,&nbsp;Chunhua Ge","doi":"10.1016/j.talo.2025.100600","DOIUrl":"10.1016/j.talo.2025.100600","url":null,"abstract":"<div><div>We report a metal-free perylene-based polyimide framework (PDI-PB) as an efficient electrocatalyst for oxygen reduction reaction (ORR)-enhanced cathodic electrochemiluminescence (ECL). PDI-PB enhances reactive oxygen species (ROS) generation via oxygen reduction that significantly amplify the luminol–dissolved oxygen ECL signal. The resulting biosensor enables ultrasensitive detection of dopamine (DA) over a wide linear range (5 × 10^–5 to 100 μM) with a record-low detection limit of 9.76 pM (S/<em>N</em> = 3). The sensor exhibits excellent selectivity, stability, and reproducibility, and was successfully applied to detect DA in human sweat, serum, and urine, yielding recoveries of 97.0–101.1 %. Density functional theory (DFT) calculations reveal that PDI-PB’s superior electronic structure facilitates efficient charge transfer and oxygen activation. This work presents a new strategy for metal-free organic electrocatalyst design, combining mechanistic insight with high-performance ECL biosensing for non-invasive health monitoring and clinical diagnostics.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100600"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an analytical method for simultaneous determination of 38 bile acids and its application in studying the mechanism of WuZhi Pian against rifampicin-induced liver injury","authors":"Caiping Zhang ,&nbsp;Jingyu Li ,&nbsp;Jianan Zheng ,&nbsp;Yiming He ,&nbsp;Yongxv Chen ,&nbsp;Fangli Liu ,&nbsp;Zhongqiu Liu ,&nbsp;Zhuoru He","doi":"10.1016/j.talo.2026.100607","DOIUrl":"10.1016/j.talo.2026.100607","url":null,"abstract":"<div><div>Bile acids (BAs) are crucial signaling molecules involved in liver metabolism regulation. The development of quantitative detection methods for BAs is essential for understanding the molecular mechanisms of liver-related diseases. In this study, we optimized an analytical method to quantify 38 BAs in serum using ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS). We evaluated and optimized various sample preparation methods, chromatographic columns, mobile phase compositions, and mass spectrometry conditions. After method validation, BA profiles were analyzed in mice with rifampicin (RIP)-induced liver injury, with or without WuZhi Pian treatment. According to the results, the optimal serum preparation involved a 1% ammonia-methanol solution at a 1:3 sample-to-solvent ratio, vortex-mixing for 5 min, and incubating for 60 min at 4°C. All 38 BAs achieved optimal separation on an ACQUITY UPLC® HSS T3 column (2.1 × 100 mm, 1.8 μm) with a 20-min run time. The best peak shapes and responses were obtained using mobile phases consisted of 4.3 mM ammonia with 0.1% acetic acid in water and acetonitrile. This method demonstrated high specificity, linearity (&gt; 0.9991), and a lower limit of quantification (LLOQ) ranging from 0.0054 to 2.4414 nM across different BAs, with good repeatability (RSD% &lt; 15.35%) and reproducibility (RSD% &lt; 19.68%). Average recoveries for all BAs ranged from 70 to 122%. Using this method, 13 BAs were found to be altered in RIP-induced liver injury, of which nine BAs were modulated by WuZhi Pian treatment. Therefore, the optimized UPLC-QQQ-MS/MS method is a powerful tool for monitoring BA metabolism-related diseases.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100607"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics and Nanoplastics in human tissues: Systematic review of evidence, analytical protocols, and methodological challenges","authors":"Simone Ottavio Zielli ,&nbsp;Jennifer Paola Pascali ,&nbsp;Antonio Mazzotti ,&nbsp;Paolo Fais ,&nbsp;Milena Fini ,&nbsp;Cesare Faldini ,&nbsp;Susi Pelotti","doi":"10.1016/j.talo.2026.100615","DOIUrl":"10.1016/j.talo.2026.100615","url":null,"abstract":"<div><div>Microplastics and NanoPlastics (MNP) have emerged as ubiquitous environmental contaminants with potential implications for human health. This systematic review synthesizes current evidence on the occurrence of MNP in human solid tissues and critically evaluates the analytical protocols employed for their detection and quantification. A comprehensive literature search conducted in September 2025 across MEDLINE, EMBASE, and the Cochrane Library, in accordance with PRISMA guidelines, identified 26 eligible studies encompassing 564 human samples from diverse biological matrices, including placenta, lung, liver, blood, and bone.</div><div>Polyethylene, polypropylene, and polyvinyl chloride were the most frequently detected polymers, while particle sizes predominantly ranged between 20 and 100 µm. Analytical techniques varied substantially across studies, with Raman and Fourier-transform infrared (FTIR) spectroscopy representing the most widely applied methods, often complemented by microscopy or pyrolysis–GC/MS for polymer confirmation. Reported MNPs abundances ranged from less than one to several thousand particles per gram of tissue, reflecting the lack of standardized procedures for extraction, quantification, and contamination control.</div><div>Recent investigations have increasingly implemented plastic-free workflows and procedural blanks, leading to improved reliability and reduced overestimation of MNP burden. Nevertheless, persistent methodological heterogeneity continues to hinder cross-study comparability and the establishment of true human tissue loads. Preliminary correlations between MNP accumulation and clinical or pathological parameters have been observed, but causal links remain unconfirmed.</div><div>This review highlights the urgent need for internationally harmonized protocols, rigorous contamination prevention, and standardized reporting to ensure reliable biomonitoring and clarify the potential health implications of microplastic exposure in humans.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100615"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stripping voltammetric analysis of cadmium on a dual working screen-printed carbon electrode modified with graphene-oxide/carbon-nanohorn composite","authors":"Watcharin Permwong ,&nbsp;Jaroon Jakmunee ,&nbsp;Somkid Pencharee","doi":"10.1016/j.talo.2026.100608","DOIUrl":"10.1016/j.talo.2026.100608","url":null,"abstract":"<div><div>Accurate field monitoring of cadmium is hindered by electrode fouling and matrix interferences that plague conventional stripping voltammetry (SV). Here we introduce a differential-SV strategy that pairs two screen-printed working electrodes on the same chip (2WEs-SPCE) to decouple pre-concentration from signal read-out. The first electrode (WE1) accumulates Cd²⁺, while the second (WE2) records a background-free, non- pre-concentrated trace; subtracting the two currents suppresses drift and eliminates false positives. Both surfaces are nanostructured with a 1:1 graphene-oxide/carbon-nanohorn (GO/CNH) film that combines GO’s affinity for heavy metals with CNH’s high conductivity, boosting adsorption kinetics and signal stability. The sensor delivers dual linear ranges, 20–300 µg. L⁻¹ (R² = 0.994; 0.0415 µA. µg⁻¹. L) and 300–1000 µg. L⁻¹ (R² = 0.986; 0.0241 µA. µg⁻¹. L), with limit of detection and limit of quantification of 4.6 and 15.20 µg. L⁻¹, respectively, appropriate for qualification of Cd²⁺ contamination in water sample. This differential 2WEs-SPCE platform marries low-cost printing with nanocarbon synergy, delivering interference-resistant, ppb-level cadmium sensing that is ready for on-site environmental and food-safety deployment.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100608"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Off - on fluorescent probe using sulfur-doped graphitic carbon nitride nanosheets for detection of copper ion and cysteine","authors":"Obwodo Omod Obang ,&nbsp;Alemensh Bekele ,&nbsp;Fuad Abduro Bushira ,&nbsp;Alemayehu Yifru ,&nbsp;Tadesse Haile Fereja ,&nbsp;Shimeles Addisu Kitte","doi":"10.1016/j.talo.2026.100618","DOIUrl":"10.1016/j.talo.2026.100618","url":null,"abstract":"<div><div>A sensitive off-on fluorescent probe was demonstrated using sulfur-doped graphitic carbon nitride nanosheets (S-g-C₃N₄ NSs) for the sequential determination of copper ions (Cu²⁺) and cysteine (Cys). The S-g-C₃N₄ NSs, synthesized via simple thermal polymerization method, exhibited strong fluorescence, which was selectively quenched by Cu²⁺ via a photoinduced electron transfer (PET) mechanism. The resulting S-g-C₃N₄/Cu²⁺complex then served as an effective platform for sensing Cys, which restored the fluorescence by competitively binding Cu²⁺. The probe demonstrated excellent sensitivity with low limit of detection (LOD) of 0.033 µM for Cu²⁺and 19 nM for Cys, high selectivity over potential interferents, and good stability. The method was successfully applied for the determination of Cu²⁺in tap water and Cys in multivitamin tablets, achieving satisfactory recoveries, which confirms its potential for practical environmental and biological monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100618"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perovskite nanocrystals as fluorescent sensors for Fe³⁺: Mechanistic insights and emerging trends","authors":"Mohamed Abu Shuheil ,&nbsp;G. PadmaPriya ,&nbsp;Subhashree Ray ,&nbsp;Talal Aziz Qassem ,&nbsp;Gunjan Garg ,&nbsp;Renu Sharma ,&nbsp;Bekzod Madaminov ,&nbsp;Sabokhat Sadikova ,&nbsp;Shayan Mahmoodi","doi":"10.1016/j.talo.2026.100616","DOIUrl":"10.1016/j.talo.2026.100616","url":null,"abstract":"<div><div>Perovskite nanocrystals (PNCs) have emerged as powerful fluorescent platforms for the selective and sensitive detection of Fe³⁺ ions due to their defect-tolerant electronic structure, high photoluminescence efficiency, and tunable optoelectronic properties. This review provides mechanistic insights into the diverse quenching pathways governing Fe³⁺ sensing, including photoinduced electron transfer, inner filter effects, cation exchange, aggregation dynamics, magnetic polaron formation, and oxidative surface processes. By correlating structural characteristics with optical responses, we highlight how composition engineering, surface chemistry, and dimensionality influence sensing performance across lead-based and lead-free systems. Emerging trends such as ratiometric emission, core–shell stabilization, hybrid organic–inorganic interfaces, and dopant-mediated enhancements are discussed as promising strategies toward improved accuracy, environmental stability, and biocompatibility. Recent advances in film-based and solid-state perovskite sensors further underscore their potential for integration into portable, low-cost detection platforms. To the best of our knowledge, this work represents the first review article specifically focused on Fe³⁺ ion sensing using PNCs, offering a unified perspective on mechanistic foundations and future directions. Overall, this work consolidates current progress while identifying challenges and opportunities for developing next-generation perovskite fluorescent sensors capable of reliable Fe³⁺ monitoring in environmental, biological, and industrial settings.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100616"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new paradigm for the discrimination of Polygonatum geographical authenticity based on the coupling of stable isotope and secondary metabolites","authors":"Xiang Zhu ,&nbsp;Qian Li ,&nbsp;Yumeng Xia ,&nbsp;Qianyu Yao ,&nbsp;Zengcai Liang ,&nbsp;Yanan Wu ,&nbsp;Yan Zhao ,&nbsp;Weijun Guan ,&nbsp;Taofeng Lu","doi":"10.1016/j.talo.2026.100610","DOIUrl":"10.1016/j.talo.2026.100610","url":null,"abstract":"<div><h3>Background</h3><div>The genus <em>Polygonatum,</em> collectively known as Polygonatum or Solomon’s Seal, contains a number of species that have edible tuber-like stems. Technology to trace the origin of harvested plant materials is crucial. Traditional stable isotope tracing utilizes C, H, O, N and even other mineral elements for cluster analysis. This method currently has some shortcomings; if the sample size is small, the resolution will decrease. Additionally, if more stable isotope contents are measured, the costs will significantly increase. <strong>It is evident that it is necessary to enhance the accuracy of origin discrimination analysis without increasing the sample size or incurring excessive costs.</strong> (101)</div></div><div><h3>Results</h3><div>Our study selected five substances: <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and polygona-polysaccharose in Polygonatum tuber-like stem to distinguish the origin of samples from six cities in southern China. The accuracy of both the original classification and cross-validation accuracy reached 100 %. The use of only four stable substances: <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H and <em>δ</em>¹⁸O has reduced the accuracy of original classification and cross-validation to 84.4 % and 75.6 %. In addition, we studied the correlation between <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and the content of polygona-polysaccharose in Polygonatum samples. There is a linear positive correlation between <em>δ</em>²H and <em>δ</em>¹⁸O from six cities in south China. The <em>δ</em>¹³C, <em>δ</em>¹⁵N, <em>δ</em>²H, <em>δ</em>¹⁸O, and the content of polygona-polysaccharose and seven environmental parameters were also tested. We found a significant positive correlation between <em>δ</em>¹⁸O vs. latitude, and a significant positive correlation between <em>δ</em>²H vs. longitude and <em>δ</em>²H vs. annual evaporation. (138)</div></div><div><h3>Significance</h3><div>Our study represents the first origin determination results of the stable isotope of Polygonatum. The accuracy and precision of origin identification using stable isotopes was enhanced by adding the content of polygona-polysaccharose even with a limited sample size. The linear relationship between the proportion of <em>δ</em>²H and <em>δ</em>¹⁸O in the tuber-like stems of Polygonatum could help us distinguish Polygonatum from other tuber foods. (63)</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100610"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of 31P NMR and principal components analysis to determine sameness or differences between diastereomeric composition of phosphorothioate oligonucleotides","authors":"Luca Gherardi ,&nbsp;Francesca Viti ,&nbsp;Salvatore Demartis ,&nbsp;Marie McNulty ,&nbsp;Giovanni Monteleone ,&nbsp;Lorenzo Di Bari","doi":"10.1016/j.talo.2026.100611","DOIUrl":"10.1016/j.talo.2026.100611","url":null,"abstract":"<div><div>Synthetic oligonucleotides often contain chemically modified backbones to prevent degradation and improve bioactivity. Phosphorothioate (PS) linkage in place of phosphodiester is the most used one. Substituting an oxygen for a sulfur atom introduces a stereogenic element on each PS, which leads to an exponentially large number of diastereomers, if the synthetic process is not stereoselective. Variable conditions during synthesis may lead to unpredictable variation in the manifold of diastereomers, with heavy fallouts in bioactivity. We developed an analytical protocol based on ³¹P-NMR followed by principal components analysis (PCA) to fingerprint each manifold. We assess the precision of this method by investigating repeatability and reproducibility. By working on two instruments at different magnetic fields, we also assess the problem of robustness and ultimately the possibility of exchanging data between laboratories.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100611"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}