Talanta Open最新文献

Ultrafast microwave-assisted green synthesis of nitrogen-doped carbon dots as turn-off fluorescent nanosensors for determination of the anticancer nintedanib: Monitoring of environmental water samples

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-20 DOI: 10.1016/j.talo.2025.100423

Galal Magdy , Asmaa Kamal El-Deen , Aya Saad Radwan , Fathalla Belal , Heba Elmansi

This study unveils a swift and sustainable approach for the synthesis of nitrogen-doped carbon quantum dots (N-CQDs) from radish leaves and urea, conducted by microwave assistance within only 50 seconds, showcasing remarkable efficiency. Comprehensive characterization confirmed the distinctive optical properties of the N-CQDs, with a quantum yield of 19.76 %, highlighting their potential as fluorescent nanosensors. The N-CQDs exhibit turn-off fluorescence properties for sensing the anticancer drug nintedanib in different environmental water samples, including hospital discharge water, sewage water, tap water, and river water. The method exhibits excellent linearity within a concentration range of 1.0–20.0 µg/mL, with a correlation coefficient exceeding 0.999 and a low detection limit down to 0.14 μg/mL. The assay of nintedanib in various environmental water samples demonstrated the applicability and sensitivity of the N-CQDs as a detection platform. The ComplexMoGAPI, AGREE, and Green Certificate-modified Eco-Scale metrics demonstrated the method's exceptional eco-friendliness and sustainability. Additionally, the BAGI tool was employed to assess the method's economic viability, applicability, and practicality. All the tools demonstrated the sustainability and feasibility of the developed approach, as well as its appropriateness for the routine analysis of the examined drug. This work is considered the first spectrofluorimetric approach for the analysis of nintedanib. This innovative study also paves the way for eco-friendly nanosensors with diverse applications in environmental monitoring.

{"title":"Ultrafast microwave-assisted green synthesis of nitrogen-doped carbon dots as turn-off fluorescent nanosensors for determination of the anticancer nintedanib: Monitoring of environmental water samples","authors":"Galal Magdy , Asmaa Kamal El-Deen , Aya Saad Radwan , Fathalla Belal , Heba Elmansi","doi":"10.1016/j.talo.2025.100423","DOIUrl":"10.1016/j.talo.2025.100423","url":null,"abstract":"<div><div>This study unveils a swift and sustainable approach for the synthesis of nitrogen-doped carbon quantum dots (N-CQDs) from radish leaves and urea, conducted by microwave assistance within only 50 seconds, showcasing remarkable efficiency. Comprehensive characterization confirmed the distinctive optical properties of the N-CQDs, with a quantum yield of 19.76 %, highlighting their potential as fluorescent nanosensors. The N-CQDs exhibit turn-off fluorescence properties for sensing the anticancer drug nintedanib in different environmental water samples, including hospital discharge water, sewage water, tap water, and river water. The method exhibits excellent linearity within a concentration range of 1.0–20.0 µg/mL, with a correlation coefficient exceeding 0.999 and a low detection limit down to 0.14 μg/mL. The assay of nintedanib in various environmental water samples demonstrated the applicability and sensitivity of the N-CQDs as a detection platform. The ComplexMoGAPI, AGREE, and Green Certificate-modified Eco-Scale metrics demonstrated the method's exceptional eco-friendliness and sustainability. Additionally, the BAGI tool was employed to assess the method's economic viability, applicability, and practicality. All the tools demonstrated the sustainability and feasibility of the developed approach, as well as its appropriateness for the routine analysis of the examined drug. This work is considered the first spectrofluorimetric approach for the analysis of nintedanib. This innovative study also paves the way for eco-friendly nanosensors with diverse applications in environmental monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100423"},"PeriodicalIF":4.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On-site trihalomethanes extraction from water using 3D printing solid phase extraction device and analysis by GC-ECD

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-18 DOI: 10.1016/j.talo.2025.100420

Diego Barzallo , Héctor Berdugo , Edwin Palacio

A 3D-printed device coated with SDB-1 resin was developed for on-site extraction and preconcentration of trihalomethanes (THMs) and subsequent determination by gas chromatography (GC) with an electron capture detector (ECD). This device was fabricated by stereolithography and covered with a commercial SPE resin using the stick & cure immobilization technique. Various parameters that affect extraction efficiency and preconcentration (resin type, conditioning mode, extraction modality, extraction time, and eluent volume) were optimized using four THMs (Chloroform, TCM; Bromodichloromethane, BDCM; Dibromochloromethane, DBCM; and Tribromomethane, TBM) and an internal standard (1,2-dibromopropane). Under the optimized conditions, good linearity for THMs studied were obtained in a range of 0.25–100 µg L⁻¹ with r ≥ 0.998 and limits of detection between 0.02–0.05 µg L⁻¹. The precision expressed as relative standard deviation (%RSD, n = 3), showed intraday and interday ranges of 1.8–4.2 % and 3.3–9.4 %, respectively, demonstrating a good precision of the proposed methodology. The proposed method was successfully applied to the simultaneous analysis of four THMs in wastewater effluent and tap water samples, revealing total concentrations of 34.1 and 78.1 µg L⁻¹, respectively. This user-friendly and miniaturized device features a simple extraction procedure (up to 24-fold enrichment), with excellent sensitivity and precision, which is very useful and practical for the simultaneous analysis of volatile compounds. Additionally, three greenness evaluation methodologies (AGREE, AGREEprep, and GAPI) and a practicality tool (BAGI) were employed to assess the environmental friendliness and applicability of the proposed method, demonstrating its superior performance compared to previously reported GC methods in the literature.

研究人员开发了一种涂有 SDB-1 树脂的三维打印装置，用于现场萃取和预浓缩三卤甲烷（THMs），然后用带有电子捕获检测器（ECD）的气相色谱法（GC）进行测定。该装置采用立体光刻技术制造，并使用粘贴&固化固定技术覆盖了商用 SPE 树脂。使用四种三卤甲烷（氯仿，TCM；溴二氯甲烷，BDCM；二溴氯甲烷，DBCM；三溴甲烷，TBM）和一种内标（1,2-二溴丙烷）对影响萃取效率和预富集的各种参数（树脂类型、调节模式、萃取方式、萃取时间和洗脱液体积）进行了优化。在优化条件下，所研究的三卤甲烷在 0.25-100 µg L-1 范围内线性关系良好，r ≥ 0.998，检出限为 0.02-0.05 µg L-1。以相对标准偏差（%RSD，n = 3）表示的日内和日间精密度范围分别为 1.8-4.2%和 3.3-9.4%，表明该方法具有良好的精密度。该方法成功地应用于同时分析废水和自来水样品中的四种三卤甲烷，结果显示总浓度分别为 34.1 和 78.1 µg L-1。该装置操作简便，体积小，萃取过程简单（富集倍数可达 24 倍），灵敏度和精密度极高，对于同时分析挥发性化合物非常实用。此外，该方法还采用了三种绿色评价方法（AGREE、AGREEprep 和 GAPI）和一种实用性工具（BAGI）来评估其环境友好性和适用性，证明其性能优于之前文献报道的气相色谱方法。

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引用次数: 0

Paper-based molecularly imprinted film designs for sensing human serum albumin

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-18 DOI: 10.1016/j.talo.2025.100421

Tzu-Chieh Lin , Chung-Yin Lin , Yi-Ting Hwang , Dar-Fu Tai

A general, simple, and inexpensive method for fast and high throughput that monitors target model proteins and the sequential incorporation of functional monomers were presented for the preparation of molecularly imprinted films (MIFs) on cellulose filter papers (MIFPs). Owing to the inaccessibility of the fatty acid binding sites on human serum albumin (HSA), four classified-segment peptides with different chain lengths (i.e., HSA_112–122, HSA_302–313, HSA_500–511, and HSA_568–582) were selected and tested with regard to both MIFPs enrichment and fluorescent detection. The novel HSA MIFPs that designed for this study involve a monomer crosslinker instead of the commonly used crosslinker. Imprinted cavities took shape in different solvent systems on MIFPs because the conformation of the template peptide is fixed. When the solvent system changed to a 70% 2,2,2-trifluoroethanol (TFE) solution, the conformation of the peptide chains changed from an extended coil structure to a helix structure. Therefore, the film bound to the mother protein selectively. The imprinting conditions were optimized while the binding affinity, adsorption kinetics, and selectivity of the MIFPs for each template peptide were also evaluated. Fluorescence assays led to generate Scatchard plots revealing that, for

{MIFP}_{568 - 582}^{Helix}

, HSA had a dissociation constant (K_d) of 4.8 μM and a maximum bind capacity (B_max) of 0.5604 nmole. The binding selectivity was 10-folds more sensitive than

{MIFP}_{568 - 582}^{Random coil}

. As for the

{MIFP}_{568 - 582}^{Helix}

response time was found to be 5 min. In short, a combined fluorescent platform could be used to monitor and capture proteins conveniently, inexpensively, quickly, and accurately detect without the need of too much professional input.

{"title":"Paper-based molecularly imprinted film designs for sensing human serum albumin","authors":"Tzu-Chieh Lin , Chung-Yin Lin , Yi-Ting Hwang , Dar-Fu Tai","doi":"10.1016/j.talo.2025.100421","DOIUrl":"10.1016/j.talo.2025.100421","url":null,"abstract":"<div><div>A general, simple, and inexpensive method for fast and high throughput that monitors target model proteins and the sequential incorporation of functional monomers were presented for the preparation of molecularly imprinted films (MIFs) on cellulose filter papers (MIFPs). Owing to the inaccessibility of the fatty acid binding sites on human serum albumin (HSA), four classified-segment peptides with different chain lengths (i.e., HSA<sub>112–122</sub>, HSA<sub>302–313</sub>, HSA<sub>500–511</sub>, and HSA<sub>568–582</sub>) were selected and tested with regard to both MIFPs enrichment and fluorescent detection. The novel HSA MIFPs that designed for this study involve a monomer crosslinker instead of the commonly used crosslinker. Imprinted cavities took shape in different solvent systems on MIFPs because the conformation of the template peptide is fixed. When the solvent system changed to a 70% 2,2,2-trifluoroethanol (TFE) solution, the conformation of the peptide chains changed from an extended coil structure to a helix structure. Therefore, the film bound to the mother protein selectively. The imprinting conditions were optimized while the binding affinity, adsorption kinetics, and selectivity of the MIFPs for each template peptide were also evaluated. Fluorescence assays led to generate Scatchard plots revealing that, for <span><math><msubsup><mtext>MIFP</mtext><mrow><mn>568</mn><mo>−</mo><mn>582</mn></mrow><mtext>Helix</mtext></msubsup></math></span>, HSA had a dissociation constant (<em>K</em><sub>d</sub>) of 4.8 μM and a maximum bind capacity (<em>B</em><sub>max</sub>) of 0.5604 nmole. The binding selectivity was 10-folds more sensitive than <span><math><msubsup><mtext>MIFP</mtext><mrow><mn>568</mn><mo>−</mo><mn>582</mn></mrow><mrow><mtext>Random</mtext><mspace></mspace><mtext>coil</mtext></mrow></msubsup></math></span>. As for the <span><math><msubsup><mtext>MIFP</mtext><mrow><mn>568</mn><mo>−</mo><mn>582</mn></mrow><mtext>Helix</mtext></msubsup></math></span> response time was found to be 5 min. In short, a combined fluorescent platform could be used to monitor and capture proteins conveniently, inexpensively, quickly, and accurately detect without the need of too much professional input.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100421"},"PeriodicalIF":4.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-performance room-temperature formic acid vapor sensors based on multi-walled carbon nanotubes/polypyrrole composites

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-06 DOI: 10.1016/j.talo.2025.100419

Rapiphun Janmanee , Wasu Phanphaisarn , Saengrawee Sriwichai , Anurat Wisitsoraat , Chaikarn Liewhiran

In this research, functionalized multi-walled carbon nanotubes/polypyrrole (fMWCNTs/PPy) composites were synthesized by in-situ oxidative polymerization approach to fabricate sensing films for room-temperature formic acid (HCOOH) detection. The material properties were characterized by X-ray spectroscopy, infrared spectroscopy, transmission electron microscopy, and nitrogen adsorption analysis. The study results confirmed the formation of well-defined multiwalled nanotubes embedded on PPy nanoparticulate matrix with varying MWCNTs loading levels. From gas-sensing measurement results, the addition of 1 %wt fMWCNTs during PPy polymerization greatly enhanced the sensor response from 2.6 to ∼900 towards 1 vol% HCOOH with excellent selectivity against H₂, CO₂, CH₄, C₂H₅OH, CH₃OH, and C₃H₆O at 25 °C. The dispersion of functionalized MWCNTs-PPy heterointerfaces could play an important role in the significant improvement of gas-sensing properties. Therefore, the functionalized MWCNTs-embedded PPy-based sensor would be an attractive choice for formic acid detection at room temperature and useful in medical and environmental applications.

{"title":"High-performance room-temperature formic acid vapor sensors based on multi-walled carbon nanotubes/polypyrrole composites","authors":"Rapiphun Janmanee , Wasu Phanphaisarn , Saengrawee Sriwichai , Anurat Wisitsoraat , Chaikarn Liewhiran","doi":"10.1016/j.talo.2025.100419","DOIUrl":"10.1016/j.talo.2025.100419","url":null,"abstract":"<div><div>In this research, functionalized multi-walled carbon nanotubes/polypyrrole (fMWCNTs/PPy) composites were synthesized by <em>in-situ</em> oxidative polymerization approach to fabricate sensing films for room-temperature formic acid (HCOOH) detection. The material properties were characterized by X-ray spectroscopy, infrared spectroscopy, transmission electron microscopy, and nitrogen adsorption analysis. The study results confirmed the formation of well-defined multiwalled nanotubes embedded on PPy nanoparticulate matrix with varying MWCNTs loading levels. From gas-sensing measurement results, the addition of 1 %wt fMWCNTs during PPy polymerization greatly enhanced the sensor response from 2.6 to ∼900 towards 1 vol% HCOOH with excellent selectivity against H<sub>2</sub>, CO<sub>2</sub>, CH<sub>4</sub>, C<sub>2</sub>H<sub>5</sub>OH, CH<sub>3</sub>OH, and C<sub>3</sub>H<sub>6</sub>O at 25 °C. The dispersion of functionalized MWCNTs-PPy heterointerfaces could play an important role in the significant improvement of gas-sensing properties. Therefore, the functionalized MWCNTs-embedded PPy-based sensor would be an attractive choice for formic acid detection at room temperature and useful in medical and environmental applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100419"},"PeriodicalIF":4.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-MIP based SPR sensor for tetracycline analysis in milk sample

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-04 DOI: 10.1016/j.talo.2025.100417

Monireh Bakhshpour-Yücel, Melike Küçük, Elif Tümay Özer, Bilgen Osman

Recent advancements in sensor technology have enabled the detection of antibiotics in food, ensuring human safety. In this study, we developed a surface plasmon resonance (SPR) sensor based on molecularly imprinted nanoparticles (MINps) for the real-time, sensitive, and in-situ detection of tetracycline (Tc). Firstly, Tc-imprinted nanoparticles (Tc-MINps) were synthesized using microemulsion polymerization. Then, the Tc-MINps were coated onto a bare gold SPR chip to develop the Tc-MINps SPR sensor. The sensor's performance was evaluated by detecting Tc in aqueous solutions. The results demonstrated a highly selective binding of Tc to the nanocavities on the surface of the Tc-MINps SPR sensor. The relationship between Tc molecules and the SPR sensor was analyzed at 0.5–20 mg/L Tc concentrations (pH 5.0). The Langmuir isotherm model was identified as the most appropriate binding model, indicating monolayer adsorption. The selectivity of the Tc-MINps SPR sensor was investigated using oxytetracycline, ciprofloxacin, and amoxicillin due to their structural similarity. The selectivity coefficients were determined as 5.54 for oxytetracycline, 23.66 for ciprofloxacin, and 28.39 for amoxicillin. Additionally, the limit of detection (LOD) for the Tc-MINps SPR sensor for Tc analysis in milk was found to be 0.45 mg/L, while the LOD for the HPLC method was 0.55 mg/L. The developed SPR sensor is suitable for Tc detection in milk due to its advantages, such as real-time monitoring, low cost, high selectivity, and reusability.

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引用次数: 0

Quinoid to benzenoid transition-driven glutathione sensing: Dual-emission carbon dots and smartphone-based ratiometric fluorescence analysis

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-03 DOI: 10.1016/j.talo.2025.100418

Al-Montaser Bellah H. Ali , Ashraf M. Mahmoud , Yousef A. Bin Jardan , Aya M. Mostafa , James Barker , Mohamed M. El-Wekil

This study presents a novel and sensitive method for the determination of glutathione (GSH), a crucial antioxidant and cellular protectant, using dual blue/orange-emitting carbon dots (BOCDs) and phenolphthalein (PHP). The sensing system operates at pH 9.0, exploiting the unique optical properties of BOCDs with emission peaks at 420 nm and 570 nm. In alkaline conditions, PHP develops a pink color that selectively quenches the 570 nm emission of the CDs while leaving the 420 nm peak unaffected. Upon introduction of GSH, the quinoid structure of PHP is converted to its benzenoid form via Michael addition, resulting in the disappearance of the pink color and subsequent restoration of the 570 nm fluorescence. This mechanism, utilized for the first time in GSH detection, offers a distinct advantage over previous methods that primarily relied on GSH's complexation capabilities. The analytical capabilities of the BOCDs/PHP ratiometric probe were extensively evaluated through multiple spectroscopic methods to understand its sensing mechanism. Performance analysis revealed impressive analytical figures of merit: the method exhibited strong linearity with a correlation coefficient of 0.9985, provided sensitive detection across a broad concentration range from 0.01 to 8.0 μM, and achieved a remarkably low detection limit of 3.33 nM. The method's versatility was enhanced through the development of a dual-mode smartphone platform, enabling both colorimetric and fluorometric GSH detection. Practical validation using human serum samples demonstrated the method's robustness in complex biological matrices, achieving high recovery rates between 98.5 % and 101.0 %, confirming its suitability for real-world clinical applications. This novel strategy combines the sensitivity of ratiometric fluorescence with the convenience of smartphone-based detection, offering a promising tool for GSH monitoring in clinical and research settings.

{"title":"Quinoid to benzenoid transition-driven glutathione sensing: Dual-emission carbon dots and smartphone-based ratiometric fluorescence analysis","authors":"Al-Montaser Bellah H. Ali , Ashraf M. Mahmoud , Yousef A. Bin Jardan , Aya M. Mostafa , James Barker , Mohamed M. El-Wekil","doi":"10.1016/j.talo.2025.100418","DOIUrl":"10.1016/j.talo.2025.100418","url":null,"abstract":"<div><div>This study presents a novel and sensitive method for the determination of glutathione (GSH), a crucial antioxidant and cellular protectant, using dual blue/orange-emitting carbon dots (BO<img>CDs) and phenolphthalein (PHP). The sensing system operates at pH 9.0, exploiting the unique optical properties of BO<img>CDs with emission peaks at 420 nm and 570 nm. In alkaline conditions, PHP develops a pink color that selectively quenches the 570 nm emission of the CDs while leaving the 420 nm peak unaffected. Upon introduction of GSH, the quinoid structure of PHP is converted to its benzenoid form via Michael addition, resulting in the disappearance of the pink color and subsequent restoration of the 570 nm fluorescence. This mechanism, utilized for the first time in GSH detection, offers a distinct advantage over previous methods that primarily relied on GSH's complexation capabilities. The analytical capabilities of the BO<img>CDs/PHP ratiometric probe were extensively evaluated through multiple spectroscopic methods to understand its sensing mechanism. Performance analysis revealed impressive analytical figures of merit: the method exhibited strong linearity with a correlation coefficient of 0.9985, provided sensitive detection across a broad concentration range from 0.01 to 8.0 μM, and achieved a remarkably low detection limit of 3.33 nM. The method's versatility was enhanced through the development of a dual-mode smartphone platform, enabling both colorimetric and fluorometric GSH detection. Practical validation using human serum samples demonstrated the method's robustness in complex biological matrices, achieving high recovery rates between 98.5 % and 101.0 %, confirming its suitability for real-world clinical applications. This novel strategy combines the sensitivity of ratiometric fluorescence with the convenience of smartphone-based detection, offering a promising tool for GSH monitoring in clinical and research settings.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100418"},"PeriodicalIF":4.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced LC-MS/MS method for selective quantification of nitrosamine impurities in Risperidone: Enhancing drug safety

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-02-01 DOI: 10.1016/j.talo.2025.100416

Hitesh Thumbar , Pankajkumar Nariya , Bhavin Dhaduk

In the current study, a simple, rapid, and sensitive analytical method was developed and validated for the determination and quantification of four nitrosamine drug substance-related impurities (NDSRIs) in Risperidone drug substance. Chromatographic separation was achieved on an ACE-5 PFP C18 column (150 mm × 4.6 mm × 3 µm) using gradient elution with 0.2 % formic acid and methanol. The method utilized a flow rate of 0.8 mL/min, an injection volume of 5 µL, and a column temperature of 40 °C. Positive electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode was used for quantification. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.12 to 0.46 and 0.23–0.93 µg/mL, respectively. The calibration curves for NDIRs impurities (0.23–185.72 µg/mL) exhibited R² values between 0.998 and 0.999. Recoveries ranged varied from 94.9 to 124.4 %, with % RSD < 15.0 %. The results indicated that the method is reliable for monitoring all NINA, NBOP, NBP, and NB impurities in Risperidone.

引用次数: 0

Sustainable and smart multi-analyte HPTLC determination of tolperisone HCl together with three pain killers using smartphone camera as a detector: Comparative study with benchtop densitometry

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-01-31 DOI: 10.1016/j.talo.2025.100415

Mohamed Rizk , Emad Ramzy , Safaa Toubar , Amr M. Mahmoud , Marwa I. Helmy

The remarkable advancement in smartphone cameras has revolutionized their potential as highly accessible, portable, and manageable detectors in various analytical approaches. In this study, we developed a novel and cost-effective method for the simultaneous determination of four drugs (Tolperisone HCl (TOLP), aceclofenac(ACEC), Paracetamol (PARA), and etodolac (ETOD)). Our innovative approach combines high-performance thin-layer chromatography (HPTLC) with a smartphone's camera. Initially, chromatographic separation has been achieved utilizing HPTLC plates, employing an environmentally friendly mobile phase consisting of a mixture of ethyl acetate, methanol, and glacial acetic acid (8.5:1.5:0.25, by volume). The developed plates were visualized using a UV lamp and captured using the rear-facing camera of a smartphone fixed onto a specially designed chamber with a UV illumination source. Using ImageJ, a freely available image analysis program, the drug spot intensities were measured throughout a concentration range of 1.0–7.0 µg/band. Furthermore, the study expanded to include a comparison between the obtained results and a conventional benchtop densitometry method at a wavelength of 276.0 nm. The densitometry method exhibited a linear response within the range of 100.0 - 700.0 ng/band for TOLP, 100.0 - 600.0 ng/band for ACEC, and 150.0 - 600.0 ng/band for PARA and ETOD. The proposed HPTLC/smartphone method demonstrated its advantages of being fast, simple, reliable, and environmentally friendly, making it an outstanding platform for analyzing commercially available combined tablets. To evaluate the environmental impact and sustainability of the constructed methods and compare them with other published methods reported in the literature, the whiteness of the developed methods utilizing the RGB 12 algorithm combined with the two highly cited greenness assessment metrics, the analytical Eco-scale (AES) and the Analytical GREEnness (AGREE), were employed.

{"title":"Sustainable and smart multi-analyte HPTLC determination of tolperisone HCl together with three pain killers using smartphone camera as a detector: Comparative study with benchtop densitometry","authors":"Mohamed Rizk , Emad Ramzy , Safaa Toubar , Amr M. Mahmoud , Marwa I. Helmy","doi":"10.1016/j.talo.2025.100415","DOIUrl":"10.1016/j.talo.2025.100415","url":null,"abstract":"<div><div>The remarkable advancement in smartphone cameras has revolutionized their potential as highly accessible, portable, and manageable detectors in various analytical approaches. In this study, we developed a novel and cost-effective method for the simultaneous determination of four drugs (Tolperisone HCl (TOLP), aceclofenac(ACEC), Paracetamol (PARA), and etodolac (ETOD)). Our innovative approach combines high-performance thin-layer chromatography (HPTLC) with a smartphone's camera. Initially, chromatographic separation has been achieved utilizing HPTLC plates, employing an environmentally friendly mobile phase consisting of a mixture of ethyl acetate, methanol, and glacial acetic acid (8.5:1.5:0.25, by volume). The developed plates were visualized using a UV lamp and captured using the rear-facing camera of a smartphone fixed onto a specially designed chamber with a UV illumination source. Using ImageJ, a freely available image analysis program, the drug spot intensities were measured throughout a concentration range of 1.0–7.0 µg/band. Furthermore, the study expanded to include a comparison between the obtained results and a conventional benchtop densitometry method at a wavelength of 276.0 nm. The densitometry method exhibited a linear response within the range of 100.0 - 700.0 ng/band for TOLP, 100.0 - 600.0 ng/band for ACEC, and 150.0 - 600.0 ng/band for PARA and ETOD. The proposed HPTLC/smartphone method demonstrated its advantages of being fast, simple, reliable, and environmentally friendly, making it an outstanding platform for analyzing commercially available combined tablets. To evaluate the environmental impact and sustainability of the constructed methods and compare them with other published methods reported in the literature, the whiteness of the developed methods utilizing the RGB 12 algorithm combined with the two highly cited greenness assessment metrics, the analytical Eco-scale (AES) and the Analytical GREEnness (AGREE), were employed.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100415"},"PeriodicalIF":4.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace analysis method for the determination of organophosphate esters based on solid-phase extraction-UPLC-MS/MS and its application to blood

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-01-25 DOI: 10.1016/j.talo.2025.100414

Luyao Hang , Lihong Duan , Lixin Yang , Hongmei Shi

Measurement of organophosphate esters (OPEs) concentrations in human body fluids is important for understanding the level of exposure to OPEs in humans and for assessing human health risks. Currently. There are more analytical tests on the metabolite levels of OPEs in urine as biomarkers of human OPEs exposure, while there are fewer studies on the analysis of OPEs in human blood. In this study, an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of 19 OPEs in human blood was developed based on the optimization of the pretreatment process and chromatographic separation. The serum samples were cleaned-up by an HMR extraction column, and the separation of the targets was performed on an Acquity BEHC18 column with a gradient elution using methanol and 5 mmol/L aqueous ammonium acetate as the mobile phases, and finally, the determination was carried out. Under the optimized detection conditions, the recoveries of the 19 OPEs ranged from 81.4 % to 117.8 % with the RSDs <20 %, and the limits of detection (LODs) were in the range of 0.024∼0.058 ng/mL. The method is simple in sample pretreatment, sensitive, and suitable for the determination of OPEs in human serum samples. The results of the analysis of human serum samples showed that the exposure of OPEs in human body is relatively common and should be widely concerned.

{"title":"Trace analysis method for the determination of organophosphate esters based on solid-phase extraction-UPLC-MS/MS and its application to blood","authors":"Luyao Hang , Lihong Duan , Lixin Yang , Hongmei Shi","doi":"10.1016/j.talo.2025.100414","DOIUrl":"10.1016/j.talo.2025.100414","url":null,"abstract":"<div><div>Measurement of organophosphate esters (OPEs) concentrations in human body fluids is important for understanding the level of exposure to OPEs in humans and for assessing human health risks. Currently. There are more analytical tests on the metabolite levels of OPEs in urine as biomarkers of human OPEs exposure, while there are fewer studies on the analysis of OPEs in human blood. In this study, an ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the determination of 19 OPEs in human blood was developed based on the optimization of the pretreatment process and chromatographic separation. The serum samples were cleaned-up by an HMR extraction column, and the separation of the targets was performed on an Acquity BEHC18 column with a gradient elution using methanol and 5 mmol/L aqueous ammonium acetate as the mobile phases, and finally, the determination was carried out. Under the optimized detection conditions, the recoveries of the 19 OPEs ranged from 81.4 % to 117.8 % with the RSDs <20 %, and the limits of detection (LODs) were in the range of 0.024∼0.058 ng/mL. The method is simple in sample pretreatment, sensitive, and suitable for the determination of OPEs in human serum samples. The results of the analysis of human serum samples showed that the exposure of OPEs in human body is relatively common and should be widely concerned.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100414"},"PeriodicalIF":4.1,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strong toxic gas detection on penta-BCP monolayers: Insights from DFT calculations

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-01-21 DOI: 10.1016/j.talo.2025.100412

Thanasee Thanasarnsurapong , Suchanuch Sringamprom , Pakpoom Reunchan , Weekit Sirisaksoontorn , Sirichok Jungthawan , Piyanooch Nedkun , Jariyanee Prasongkit , Adisak Boonchun

Rapid and accurate detection of toxic gases remains important for industrial safety and environmental well-being. This study examines the potential of penta-BCP monolayer as a high-performance toxic gas sensor utilizing the robust framework of density functional theory (DFT). Penta-BCP exhibits strong and selective interactions with specific toxic gas molecules, including CO, NO, and

{NO}_{2}

. This results in the remarkable ability to readily capture these harmful gases while demonstrating minimal interaction with non-toxic counterparts such as

H_{2}

,

N_{2}

,

{CO}_{2}

, and

{CH}_{4}

, underlining its exceptional selectivity. Furthermore, penta-BCP has a significantly shorter recovery time for NO and

{NO}_{2}

gases compared to penta-BCN, which has a similar structure. These findings collectively position penta-BCP as a frontrunner for the next generation of toxic gas sensors, paving the way for enhanced environmental monitoring and improved public health protection.

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引用次数: 0