{"title":"Optical twisting to monitor the rheology of single cells.","authors":"Matthieu Robert de Saint Vincent","doi":"10.3233/BIR-15084","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Biological cells exhibit complex mechanical properties which determine their responses to applied force.</p><p><strong>Objective: </strong>We developed an optical method to probe the temporal evolution of power-law rheology of single cells.</p><p><strong>Methods: </strong>The method consisted in applying optically a constant mechanical torque to a birefringent microparticle bound to the cell membrane, and observing dynamics of the particle's in-plane rotation.</p><p><strong>Results: </strong>The deformation dynamics of the membrane followed a power law of time, which directly relates to cytoskeletal prestress as reported in the literature. The temporal evolution of this rheological behaviour, over time scales of several minutes, showed strong variations of the exponent on single adherent cells not subject to any specific treatment.</p><p><strong>Conclusions: </strong>The consistent observation of variations in the exponent suggests that, in their normal activity, living cells modulate their prestress by up to three orders of magnitude within minutes.</p>","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"53 2","pages":"69-80"},"PeriodicalIF":1.0000,"publicationDate":"2016-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-15084","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biorheology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3233/BIR-15084","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 1
Abstract
Background: Biological cells exhibit complex mechanical properties which determine their responses to applied force.
Objective: We developed an optical method to probe the temporal evolution of power-law rheology of single cells.
Methods: The method consisted in applying optically a constant mechanical torque to a birefringent microparticle bound to the cell membrane, and observing dynamics of the particle's in-plane rotation.
Results: The deformation dynamics of the membrane followed a power law of time, which directly relates to cytoskeletal prestress as reported in the literature. The temporal evolution of this rheological behaviour, over time scales of several minutes, showed strong variations of the exponent on single adherent cells not subject to any specific treatment.
Conclusions: The consistent observation of variations in the exponent suggests that, in their normal activity, living cells modulate their prestress by up to three orders of magnitude within minutes.
期刊介绍：
Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials.
The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.
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