Mouse Models of Asthma

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2016-06-01 DOI:10.1002/cpmo.4

Nincy Debeuf, Eline Haspeslagh, Mary van Helden, Hamida Hammad, Bart N. Lambrecht

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引用次数: 64

Abstract

Allergic asthma is a chronic inflammatory disease of the conducting airways characterized by the presence of allergen-specific IgE, Th2 cytokine production, eosinophilic airway inflammation, bronchial hyperreactivity, mucus overproduction, and structural changes in the airways. Investigators have tried to mimic these features of human allergic asthma in murine models. Whereas the surrogate allergen ovalbumin has been extremely valuable for unravelling underlying mechanisms of the disease, murine asthma models depend nowadays on naturally occurring allergens, such as house dust mite (HDM), cockroach, and Alternaria alternata. Here we describe a physiologically relevant model of acute allergic asthma based on sensitization and challenge with HDM extracts, and compare it with the ovalbumin/alum-induced asthma model. Moreover, we propose a detailed readout of the asthma phenotype, determining the degree of eosinophilia in bronchoalveolar lavage fluids by flow cytometry, visualizing goblet cell metaplasia, and measuring Th cytokine production by lung-draining mediastinal lymph node cells restimulated with HDM. © 2016 by John Wiley & Sons, Inc.

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哮喘小鼠模型

过敏性哮喘是一种传导气道的慢性炎症性疾病，其特征是存在过敏原特异性IgE、Th2细胞因子产生、嗜酸性气道炎症、支气管高反应性、粘液过量产生和气道结构改变。研究人员试图在小鼠模型中模仿人类过敏性哮喘的这些特征。虽然替代过敏原卵清蛋白在揭示疾病的潜在机制方面非常有价值，但现在的小鼠哮喘模型依赖于自然发生的过敏原，如屋尘螨(HDM)、蟑螂和交替孢霉。在这里，我们描述了一个基于HDM提取物致敏和激发的急性过敏性哮喘生理学相关模型，并将其与卵清蛋白/铝诱导的哮喘模型进行比较。此外，我们提出了哮喘表型的详细读数，通过流式细胞术确定支气管肺泡灌洗液中嗜酸性粒细胞增多的程度，观察杯状细胞化生，并通过HDM重新刺激肺引流纵隔淋巴结细胞测量Th细胞因子的产生。©2016 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.