{"title":"Issue Information","authors":"","doi":"10.1002/cpmo.73","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: In Bassiouni et al. (http://doi.org/10.1002/cpmo.84), the image shows frozen sections of the C57BL/6J mouse cochlea at p3. To demonstrate the “mouse-on-mouse” background, we omitted incubation with the primary antibody but incubated the sections only with a secondary antibody (goat anti-mouse Alexa Fluor 488). Some slides were blocked with 5% normal goat serum for 30 min. Other slides underwent the same serum blocking step, followed by a second blocking step with an anti-mouse-IgG blocking reagent for 1 hr at RT. (<b>A</b>) Using sole blocking with serum resulted in a strong background fluorescence signal, most prominently in the spiral limbus, and a lateral cochlear wall. A moderate signal was seen in the basilar membrane, intercellular borders, spiral lamina, and in the capillaries of the stria vascularis. (<b>B</b>) Using double-blocking resulted in no background fluorescence signal under identical exposure time and microscopy conditions. Scale: 20 µm.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"10 4","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.73","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in mouse biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmo.73","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Cover: In Bassiouni et al. (http://doi.org/10.1002/cpmo.84), the image shows frozen sections of the C57BL/6J mouse cochlea at p3. To demonstrate the “mouse-on-mouse” background, we omitted incubation with the primary antibody but incubated the sections only with a secondary antibody (goat anti-mouse Alexa Fluor 488). Some slides were blocked with 5% normal goat serum for 30 min. Other slides underwent the same serum blocking step, followed by a second blocking step with an anti-mouse-IgG blocking reagent for 1 hr at RT. (A) Using sole blocking with serum resulted in a strong background fluorescence signal, most prominently in the spiral limbus, and a lateral cochlear wall. A moderate signal was seen in the basilar membrane, intercellular borders, spiral lamina, and in the capillaries of the stria vascularis. (B) Using double-blocking resulted in no background fluorescence signal under identical exposure time and microscopy conditions. Scale: 20 µm.
期刊介绍：
Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.
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