Laminin- and basement membrane-polycaprolactone blend nanofibers as a scaffold for regenerative medicine.

Nanomaterials and the environment Pub Date : 2014-09-01 DOI:10.2478/nanome-2014-0001

Rebekah A Neal, Steven M Lenz, Tiffany Wang, Daniel Abebayehu, Benjamin P C Brooks, Roy C Ogle, Edward A Botchwey

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引用次数: 13

Abstract

Mimicking one or more components of the basement membrane (BM) holds great promise for overcoming insufficiencies in tissue engineering therapies. We have electrospun laminin nanofibers (NFs) isolated from the murine Engelbreth-Holm Swarm (EHS) tumor and evaluated them as a scaffold for embryonic stem cell culture. Seeded human embryonic stem cells were found to better maintain their undifferentiated, colony environment when cultured on laminin NFs compared to laminin mats, with 75% remaining undifferentiated on NFs. Mouse embryonic stem cells cultured on 10% laminin-polycaprolactone (PCL) NFs maintained their colony formation for twice as long without passage compared to those on PCL or gelatin substrates. In addition, we have established a protocol for electrospinning reconstituted basement membrane aligned (RBM)-PCL NFs within 10° of angular deviation. Neuron-like PC12 cells show significantly greater attachment (p < 0.001) and percentage of neurite-extending cells in vitro on 10% RBM-PCL NFs when compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p < 0.001, respectively). Together, these results implicate laminin- and RBM-PCL scaffolds as a promising biomimetic substrate for regenerative medicine applications.

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层粘连蛋白和基底膜-聚己内酯共混纳米纤维作为再生医学支架。

模拟基底膜(BM)的一种或多种成分对于克服组织工程治疗的不足具有很大的希望。我们从小鼠恩格尔布雷斯-霍尔姆群(EHS)肿瘤中分离出电纺丝层粘连蛋白纳米纤维(NFs)，并对其作为胚胎干细胞培养的支架进行了评价。与层粘连蛋白垫相比，在层粘连蛋白NFs上培养的人胚胎干细胞能更好地保持未分化的集落环境，75%的人胚胎干细胞在NFs上保持未分化。小鼠胚胎干细胞在10%层粘连蛋白-聚己内酯(PCL) NFs上培养时，其菌落形成的时间比在PCL或明胶基质上培养的时间长两倍。此外，我们还建立了在10°角偏差范围内的静电纺丝重组基膜排列(RBM)-PCL NFs的方案。与1%和0% RBM-PCL NFs相比，10% RBM-PCL NFs上的神经元样PC12细胞的附着(p < 0.001)和神经突延伸细胞的体外百分比显著增加(p < 0.015和p < 0.001)。综上所述，这些结果表明层粘连蛋白和RBM-PCL支架是一种很有前途的再生医学仿生底物。

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