Incidence and detection of beak and feather disease virus in psittacine birds in the UAE

Q1 Biochemistry, Genetics and Molecular Biology Biomolecular Detection and Quantification Pub Date : 2016-01-01 DOI:10.1016/j.bdq.2015.10.001

F. Hakimuddin , F. Abidi , O. Jafer , C. Li , U. Wernery , Ch. Hebel , K. Khazanehdari

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引用次数: 14

Abstract

Beak and feather disease is caused by Circovirus, which affects actively growing beak and feather cells of avian species. The disease affects mainly young birds while older birds may overcome the disease with few lasting effects. Due to lack of treatment, the only way to control the disease is through hygiene and early diagnosis. As a diagnostic tool, we have established a Taqman probe based real-time PCR assay to detect the presence of the viral genome in psittacine birds in UAE and reported the incidence of circovirus in different species of psittacine birds. The sensitivity of our assay was found to be very high with detection limit of up to 3.5 fg of DNA in the sample. The mean prevalence of circovirus was found to be 58.33% in African Grey Parrots, 34.42% in Cockatoos, 31.8% in amazon parrots and 25.53% in Macaws.

The Taqman assay is a quick, reliable and sensitive detection method that has been instrumental in identifying this disease that was not previously reported in the region.

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阿联酋雀鸟喙羽病病毒的发病率及检测

喙羽病是一种由环状病毒引起的疾病，主要影响禽类禽类活跃生长的喙羽细胞。这种疾病主要影响雏鸟，而年长的鸟类可能会克服这种疾病，几乎没有持久的影响。由于缺乏治疗，控制这种疾病的唯一途径是通过卫生和早期诊断。作为诊断工具，我们建立了基于Taqman探针的实时PCR检测阿联酋雀鸟中病毒基因组的存在，并报道了不同种类雀鸟中圆环病毒的发病率。我们的检测方法灵敏度很高，样品中DNA的检出限高达3.5 fg。非洲灰鹦鹉、凤头鹦鹉、亚马逊鹦鹉和金刚鹦鹉的圆环病毒平均感染率分别为58.33%、34.42%、31.8%和25.53%。Taqman试验是一种快速、可靠和敏感的检测方法，在确定该地区以前未报告的这种疾病方面发挥了重要作用。

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