Establishing essential quality criteria for the validation of circular RNAs as biomarkers

Q1 Biochemistry, Genetics and Molecular Biology Biomolecular Detection and Quantification Pub Date : 2019-03-01 DOI:10.1016/j.bdq.2019.100085

Christina Pfafenrot, Christian Preußer

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引用次数: 11

Abstract

Non-coding RNAs were established in the last decade as a new valuable biomarker class for human diseases. Specifically, circular RNAs (circRNAs) were only recently discovered as a new large group of non-coding RNAs that, due to their circular configuration, are metabolically more stable compared to their linear counterparts and therefore highly suitable for biomarker use. Based on high-throughput sequencing, the catalogs of endogenous circRNAs with disease relevance and correlation continue to grow steadily. As a consequence, circRNAs emerged as novel and attractive biomarkers, indicated by numerous recent publications. Here we would like to stress the need of essential quality criteria for validation and characterization of circular RNAs. In addition to high-throughput sequencing, classical biochemical methods are essential and should be applied for the characterization of this special class of RNAs, in particular to convincingly confirm their circularity.

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建立验证环状rna作为生物标志物的基本质量标准

非编码rna是近十年来作为一种新的有价值的人类疾病生物标志物类别而建立起来的。具体来说，环状rna (circRNAs)是最近才被发现的一种新的大型非编码rna，由于它们的环状结构，与线性rna相比，它们在代谢方面更稳定，因此非常适合用于生物标志物。基于高通量测序，具有疾病相关性和相关性的内源性环状rna目录继续稳步增长。因此，许多最近的出版物表明，环状rna成为了新颖而有吸引力的生物标志物。在这里，我们想强调需要基本的质量标准来验证和表征环状rna。除了高通量测序外，经典的生化方法也是必不可少的，应该应用于这类特殊rna的表征，特别是令人信服地证实它们的环状性。

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