[Evaluation of the Recombinant Protein Tp0965 of Treponema Pallidum as Perspective Antigen for the Improved Serological Diagnosis of Syphilis].

A V Runina, A S Starovoitova, D G Deryabin, A A Kubanov
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引用次数: 5

Abstract

Unlabelled: BACKGRAUND. Treponemal tests based on the detection of antibodies against the Treponema pallidum antigens are the most specific methods for serological diagnosis of syphilis. Due to the inability to cultivate this bacterium in vitro, the most promising sources of antigens for diagnostics are recombinant proteins of T. pallidum. Evaluation of the analytical value of certain T. pallidum proteins is the approach to improve sensitivity, specificity, and reproducibility of syphilis serological tests, including possibilities of differential diagnosis of various forms of the disease.

Objective: The aim of the research was to evaluate the analytical values (sensitivity and specificity) of recombinant protein Tp0965 of T. pallidum as a candidate antigen for serological diagnosis of syphilis.

Methods: tp0965 gene was cloned into the expression vector pET28a and the construct was used for the transformation of E. coli BL-21 (DE3) cells and further expression and purification of the recombinant protein. The collected protein was used as T. pallidum antigen for serum analysis (ELISA) of groups of patients with various forms of syphilis (n=84) and the group of healthy donors (n = 25).

Results: High frequency of positive ELISA results was shown with serum of patients with syphilis, compared to the group of healthy donors. The sensitivity of serological reactions using recombinant protein Tp0965 was 98.8%, specificity--87.5%. The highest sensitivity (100%) was detected in the groups of patients with primary, secondary and early latent syphilis while in the group of patients with late latent syphilis it decreased to 95.2%.

Conclusions: We concluded that due to its specificity T. pallidum recombinant protein Tp0965 can be used as a novel perspective antigen for development of syphilis serological diagnostic assays (for primary and early latent forms).

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梅毒螺旋体重组蛋白Tp0965在梅毒血清学诊断中的价值
未标记的:BACKGRAUND。基于梅毒螺旋体抗原抗体检测的梅毒螺旋体试验是梅毒血清学诊断最具体的方法。由于无法在体外培养这种细菌,最有希望的诊断抗原来源是T. pallidum的重组蛋白。评价某些梅毒t蛋白的分析价值是提高梅毒血清学试验的敏感性、特异性和可重复性的方法,包括对各种形式的疾病进行鉴别诊断的可能性。目的:探讨梅毒T. pallidum重组蛋白Tp0965作为梅毒血清学诊断候选抗原的分析价值(敏感性和特异性)。方法:将tp0965基因克隆到表达载体pET28a中,利用该构建体转化大肠杆菌bl21 (DE3)细胞,进一步表达纯化重组蛋白。收集的蛋白作为梅毒t抗原,分别用于84例不同类型梅毒患者和25例健康献血者的血清分析(ELISA)。结果:梅毒患者血清ELISA阳性的频率高于健康献血者。重组蛋白Tp0965的血清学反应敏感性为98.8%,特异性为87.5%。原发性、继发性和早期潜伏性梅毒患者的敏感性最高(100%),而晚期潜伏性梅毒患者的敏感性降至95.2%。结论:由于梅毒T.重组蛋白Tp0965的特异性,我们认为它可以作为一种新的前景抗原,用于梅毒(原发性和早期潜伏型)的血清学诊断。
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来源期刊
CiteScore
1.50
自引率
0.00%
发文量
31
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