Non-Typeable Haemophilus influenzae Infection of the Junbo Mouse

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2017-03-02 DOI:10.1002/cpmo.24

Michael T. Cheeseman, Derek W. Hood

引用次数: 2

Abstract

Acute otitis media, inflammation of the middle ear bulla, is the most common bacterial infection in children. For one of the principal otopathogens, non-typeable Haemophilus influenzae (NTHi), animal models allow us to investigate host-microbial interactions relevant to the onset and progression of infection and to study treatment of middle ear disease. We have established a robust model of NTHi middle ear infection in the Junbo mouse. Intranasal inoculation with NTHi produces high rates of bulla infection and high bacterial titers in bulla fluids; bacteria can also spread down the respiratory tract to the mouse lung. An innate immune response is detected in the bulla of Junbo mice following NTHi infection, and bacteria are maintained in some ears at least up to day 56 post-inoculation. The Junbo/NTHi infection model facilitates studies on bacterial pathogenesis and antimicrobial intervention regimens and vaccines for better treatment and prevention of NTHi middle ear infection. © 2017 by John Wiley & Sons, Inc.

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君波小鼠不可分型流感嗜血杆菌感染

急性中耳炎，即中耳球囊的炎症，是儿童最常见的细菌感染。对于主要的耳病原体之一，不可分型的流感嗜血杆菌(NTHi)，动物模型使我们能够研究与感染发生和进展相关的宿主-微生物相互作用，并研究中耳疾病的治疗。我们建立了君波小鼠NTHi中耳感染的稳健模型。鼻内接种NTHi可产生高球囊感染率和球囊液中高细菌滴度;细菌也可以通过呼吸道传播到老鼠的肺部。在NTHi感染后，君波小鼠的大球囊中检测到先天性免疫反应，并且在接种后至少56天，细菌在一些耳朵中保持不变。Junbo/NTHi感染模型有助于研究细菌致病机制和抗菌干预方案和疫苗，以更好地治疗和预防NTHi中耳感染。©2017 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.