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{"title":"Measuring Protein Interactions by Optical Biosensors.","authors":"Huaying Zhao, Lisa F Boyd, Peter Schuck","doi":"10.1002/cpps.31","DOIUrl":null,"url":null,"abstract":"<p><p>This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is placed on description of robust approaches that will provide reliable results with few assumptions. How to avoid the most commonly encountered problems and artifacts is also discussed. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":10866,"journal":{"name":"Current Protocols in Protein Science","volume":"88 ","pages":"20.2.1-20.2.25"},"PeriodicalIF":0.0000,"publicationDate":"2017-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpps.31","citationCount":"24","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Protein Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/cpps.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 24
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Abstract
This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is placed on description of robust approaches that will provide reliable results with few assumptions. How to avoid the most commonly encountered problems and artifacts is also discussed. © 2017 by John Wiley & Sons, Inc.
光学生物传感器测量蛋白质相互作用。
本单元介绍了用于测量可逆蛋白质相互作用平衡和动力学的光学生物传感的基本技术。重点放在描述健壮的方法，将提供可靠的结果与很少的假设。还讨论了如何避免最常遇到的问题和工件。©2017 by John Wiley & Sons, Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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