{"title":"Issue Information","authors":"","doi":"10.1002/cpps.91","DOIUrl":null,"url":null,"abstract":"<p><b>Cover</b>: In Welch et al. (http://doi.org/10.1002/cpps.113), the image shows schematic presentation of the capture and release (CaRe) method. <b>1</b>, crude extracts. <b>2</b>, crude extracts mixed with a target capturing agent (TCA). <b>3</b>, TCA captures target (lectin or glycoprotein) by binding and cross-linking the target. <b>4</b>, after centrifugation, the supernatant containing all the impurities is discarded. Only the target-TCA complex remains in the test tube. <b>5</b>, monovalent ligand (ML) is added to the complex. <b>6</b>, ML dissolves the complex and releases the target. <b>7</b>, the released target is separated from the TCA by filtration.\n\n <figure>\n <div><picture>\n <source></source></picture><p></p>\n </div>\n </figure></p>","PeriodicalId":10866,"journal":{"name":"Current Protocols in Protein Science","volume":"101 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpps.91","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Protein Science","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpps.91","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cover: In Welch et al. (http://doi.org/10.1002/cpps.113), the image shows schematic presentation of the capture and release (CaRe) method. 1, crude extracts. 2, crude extracts mixed with a target capturing agent (TCA). 3, TCA captures target (lectin or glycoprotein) by binding and cross-linking the target. 4, after centrifugation, the supernatant containing all the impurities is discarded. Only the target-TCA complex remains in the test tube. 5, monovalent ligand (ML) is added to the complex. 6, ML dissolves the complex and releases the target. 7, the released target is separated from the TCA by filtration.
期刊介绍：
With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
