A Multi-Parametric Fluorescent Assay for the Screening and Mechanistic Study of Drug-Induced Steatosis in Liver Cells in Culture

Laia Tolosa, M. José Gómez-Lechón, M. Teresa Donato
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引用次数: 1

Abstract

Human hepatic cells have been used for drug safety risk evaluations throughout early development phases. They provide rapid, cost-effective early feedback to identify drug candidates with potential hepatotoxicity. This unit presents a cell-based assay to evaluate the risk of liver damage associated with steatogenic drugs. Detailed protocols for cell exposure to test compounds and for the assessment of steatosis-related cell parameters (intracellular lipid content, reactive oxygen species production, mitochondrial impairment, and cell death) are provided. A few representative results that illustrate the utility of this procedure for the screening of drug-induced steatosis are shown. © 2017 by John Wiley & Sons, Inc.

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多参数荧光法筛选培养肝细胞药物性脂肪变性及机制研究
人类肝细胞已被用于药物早期开发阶段的安全性风险评估。它们提供了快速、具有成本效益的早期反馈,以识别具有潜在肝毒性的候选药物。本单元提出了一种以细胞为基础的测定方法,以评估与致脂肪性药物相关的肝损伤风险。提供了细胞暴露于测试化合物和脂肪变性相关细胞参数(细胞内脂质含量、活性氧产生、线粒体损伤和细胞死亡)评估的详细方案。一些有代表性的结果,说明该程序的效用筛选药物引起的脂肪变性显示。©2017 by John Wiley &儿子,Inc。
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