Detection of Human Polyomavirus DNA Using the Genome Profiling Method.

The Open Virology Journal Pub Date : 2015-11-24 eCollection Date: 2015-01-01 DOI:10.2174/1874357901509010029
Yuka Tanaka, Rieko Hirata, Kyohei Mashita, Stuart Mclean, Hiroshi Ikegaya
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引用次数: 2

Abstract

Background: In the field of forensic medicine, it is very difficult to know prior to autopsy what kind of virus has infected a body.

Objective: We assessed the potential of the genome profiling (GP) method, which was developed in the field of bioengineering, to identify viruses belonging to one species.

Method: Two species in the same family, JC and BK viruses, were used in this study. Using plasmid samples, we compared the findings of molecular phylogenetic analysis using conventional genome sequencing with the results of cluster analysis using the random PCR-based GP method and discussed whether the GP method can be used to determine viral species.

Results: It was possible to distinguish these two different viral species. In addition to this, in our trial we could also detect the JC virus from a clinical sample.

Conclusion: This method does not require special reagent sets for each viral species. Though our findings are still in the trial period, the GP method may be a simple, easy, and economical tool to detect viral species in the near future.

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用基因组谱法检测人多瘤病毒DNA。
背景:在法医学领域,在尸检之前很难知道是哪种病毒感染了尸体。目的:研究生物工程领域发展起来的基因组分析(GP)方法在同一物种病毒鉴定中的应用潜力。方法:以同一科的JC病毒和BK病毒为研究对象。利用质粒样本,我们比较了传统基因组测序的分子系统发育分析结果与基于随机pcr的GP方法的聚类分析结果,并讨论了GP方法是否可以用于确定病毒种类。结果:可以区分这两种不同的病毒。除此之外,在我们的试验中,我们还可以从临床样本中检测到JC病毒。结论:该方法不需要对每种病毒都使用专用试剂。虽然我们的发现仍处于试验阶段,但在不久的将来,GP方法可能是一种简单、容易和经济的检测病毒物种的工具。
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