Genomics insights into production of 2-methylisoborneol and a putative cyanobactin by Planktothricoides sp. SR001.

Q3 Biochemistry, Genetics and Molecular Biology Standards in Genomic Sciences Pub Date : 2017-06-05 eCollection Date: 2017-01-01 DOI:10.1186/s40793-017-0247-1

Shu Harn Te, Boon Fei Tan, Chek Yin Boo, Janelle Renee Thompson, Karina Yew-Hoong Gin

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Abstract

Planktothricoides is a free-living filamentous cyanobacterium belonging to the order Oscillatoriales and the family Phormidiaceae, capable of forming bloom in fresh and brackish waters. A unicyanobacterial non-axenic culture dominated by Planktothricoides sp. SR001 was obtained from a freshwater reservoir in Singapore. The draft genome presented here is the first tropical freshwater Planktothricoides sp. ever sequenced. The genome of 7.0Mbp contains 5,776 genes predicted using the JGI IMG pipeline. The whole genome sequence allows identification of genes encoding for nitrogen-fixation, accessory photosynthetic pigments and biosynthesis of an off-flavor compound, 2-methylisoborneol, which has been experimentally verified here based on metabolite detection. In addition, strain SR001 genome contains an operon putatively involved in the production of a linear tripeptide cyanobactin related to viridisamide A and aeruginosamide, with the later known to possess anti-microbial or cytotoxic effect.

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浮游三栖植物sp. SR001生产2-甲基异龙脑和一种推定的蓝藻素的基因组学见解。

浮游蓟藻是一种自由生活的丝状蓝藻，属于振荡目和蓝藻科，能够在淡水和咸淡水中形成水华。从新加坡的一个淡水水库中获得了一株以浮游蓟藻sp. SR001为主要菌株的单胞菌非无菌培养物。这里提出的基因组草案是热带淡水浮游三纲纲动物的第一个测序。使用JGI IMG管道预测的7.0Mbp基因组包含5776个基因。整个基因组序列允许鉴定编码固氮，辅助光合色素和恶臭化合物2-甲基异龙脑的生物合成的基因，这已经在代谢检测的基础上得到了实验验证。此外，菌株SR001基因组含有一个操纵子，据推测，该操纵子参与了与病毒二酰胺a和铜绿糖酰胺相关的线状三肽cyanobactin的产生，后者已知具有抗微生物或细胞毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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