Mouse Model of Burn Wound and Infection: Thermal (Hot Air) Lesion-Induced Immunosuppression

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2017-06-19 DOI:10.1002/cpmo.25

Henrik Calum, Niels Høiby, Claus Moser

引用次数: 15

Abstract

The immunosuppression induced by thermal injury renders the burned victim susceptible to infection. A mouse model was developed to examine the immunosuppression, which was possible to induce even at a minor thermal insult of 6% total body surface area. After induction of the burn (48 hr) a depression of leukocytes in the peripheral blood was found of the burned mice. This depression was due to a reduction in the polymorphonuclear cells. The burned mice were not able to clear a Pseudomonas aeruginosa wound infection, since the infection spread to the blood as compared to mice only infected with P. aeruginosa subcutaneously. The burn model offers an opportunity to study infections under these conditions. The present model can also be used to examine new antibiotics and immune therapy. Our animal model resembling the clinical situation is useful in developing new treatments of burn wound victims. © 2017 by John Wiley & Sons, Inc.

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烧伤创面和感染小鼠模型:热(热空气)损伤诱导的免疫抑制

热损伤引起的免疫抑制使烧伤患者易受感染。我们建立了小鼠模型来检测免疫抑制，即使在6%体表面积的轻微热损伤下也可能诱导免疫抑制。烧伤诱导48小时后，发现烧伤小鼠外周血白细胞下降。这种抑制是由于多形核细胞的减少。烧伤小鼠不能清除铜绿假单胞菌伤口感染，因为与仅皮下感染铜绿假单胞菌的小鼠相比，感染扩散到血液中。烧伤模型为研究这些条件下的感染提供了机会。该模型也可用于检验新的抗生素和免疫疗法。我们的动物模型与临床情况相似，有助于开发烧伤伤员的新治疗方法。©2017 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.