Chemical Composition and Cytotoxic and Antibacterial Activities of the Essential Oil of Aloysia citriodora Palau Grown in Morocco.

Q1 Pharmacology, Toxicology and Pharmaceutics Advances in Pharmacological Sciences Pub Date : 2017-01-01 Epub Date: 2017-06-12 DOI:10.1155/2017/7801924

Moulay Ali Oukerrou, Mounir Tilaoui, Hassan Ait Mouse, Inass Leouifoudi, Abdeslam Jaafari, Abdelmajid Zyad

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引用次数: 33

Abstract

The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas chromatography-mass spectrometry analysis. The cytotoxic activity against P815, MCF7, and VERO cell lines as well as the normal human peripheral blood mononuclear cells (PBMCs) was evaluated using the MTT assay. Standard, ATCC, strains of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) were cultivated in Muller Hinton media. Then, agar disc diffusion, minimum inhibitory concentrations (MICs), and minimal bactericidal concentrations (MBCs) were determined using microdilution method. The essential oils obtained were predominantly composed of β-spathulenol (15.61%), Ar-curcumene (14.15%), trans-caryophyllene oxide (14.14%), and neral (10.02%). The results of the assays showed that the cytotoxic effect of the essential oil of A. citriodora was high on P815 and moderate on MCF7 and on VERO cell lines. However, no cytotoxic effect was observed on PBMCs. On the other hand, essential oils showed a significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. MICs ranged between 2.84 and 8.37 mg/ml. Essential oil of A. citriodora leaves possesses significant antibacterial effect and cytotoxic activity against tumor cell lines.

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摩洛哥产金缕荆挥发油的化学成分、细胞毒及抗菌活性。

这项工作的目的是研究在摩洛哥不同地区收获的芦荟精油的体外细胞毒和抗菌作用。采用气相色谱-质谱联用分析方法建立化学谱图。采用MTT法评价其对P815、MCF7和VERO细胞系以及正常人外周血单个核细胞(PBMCs)的细胞毒活性。标准，ATCC，细菌菌株(大肠杆菌，金黄色葡萄球菌和铜绿假单胞菌)在Muller Hinton培养基中培养。然后用微量稀释法测定琼脂盘扩散、最低抑菌浓度(mic)和最低杀菌浓度(MBCs)。得到的精油主要由β-spathulenol(15.61%)、Ar-curcumene(14.15%)、trans-石竹烯氧化物(14.14%)和neral(10.02%)组成。结果表明，香茅精油对P815细胞株的细胞毒作用较高，对MCF7和VERO细胞株的细胞毒作用中等。然而，未观察到对PBMCs的细胞毒性作用。另一方面，精油对革兰氏阴性菌和革兰氏阳性菌均表现出显著的抗菌活性。mic介于2.84至8.37 mg/ml之间。香茅叶精油对肿瘤细胞具有明显的抑菌作用和细胞毒活性。

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来源期刊

Advances in Pharmacological Sciences PHARMACOLOGY & PHARMACY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

14 weeks

期刊介绍： Advances in Pharmacological and Pharmaceutical Sciences is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of experimental and clinical pharmacology, pharmaceutics, medicinal chemistry and drug delivery. Topics covered by the journal include, but are not limited to: -Biochemical pharmacology, drug mechanism of action, pharmacodynamics, pharmacogenetics, pharmacokinetics, and toxicology. -The design and preparation of new drugs, and their safety and efficacy in humans, including descriptions of drug dosage forms. -All areas of medicinal chemistry, such as drug discovery, design and synthesis. -Basic biology of drug and gene delivery through to application and development of these principles, through therapeutic delivery and targeting. Areas covered include bioavailability, controlled release, microcapsules, novel drug delivery systems, personalized drug delivery, and techniques for passing biological barriers.