miR-19a and miR-20a and Tissue Factor Expression in Activated Human Peripheral Blood Mononuclear Cells.

Thrombosis Pub Date : 2017-01-01 Epub Date: 2017-10-30 DOI:10.1155/2017/1076397

Cristina Balia, Mirella Giordano, Valentina Scalise, Tommaso Neri, Gabriella Fontanini, Fulvio Basolo, Alessandro Celi, Roberto Pedrinelli

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引用次数: 5

Abstract

Background and aims: To investigate the behaviour of miR-19a and miR-20a, two microRNAs involved in posttranscriptional modulation of TF expression in peripheral blood mononuclear cells (PBMCs) exposed to high glucose (HG) and lipopolysaccharide (LPS), and to evaluate the involvement of angiotensin II in that process.

Methods: TF Procoagulant Activity (PCA, one-stage clotting assay), antigen (Ag, ELISA), and miR-19a and miR-20a levels (specific TaqMan® MicroRNA Assays) were evaluated in PBMCs exposed to high glucose (HG, 50 mM), LPS (100 ng/mL), and Olmesartan (OLM, 10^-6 M), an angiotensin II type 1 receptor antagonist.

Results: HG increased TF expression and decreased both miRs as compared to control glucose conditions (11.1 mM). In HG-activated PBMCs, LPS stimulated TF expression and downregulated miR-20a, an effect reverted by OLM (10^-6 M); miR-19a expression was unchanged by LPS in both CG and HG conditions.

Conclusions: miR-19a and miR-20a are inhibited by inflammatory stimuli active on TF expression and their response differs by the stimulus under investigation; angiotensin II may participate in that mechanism.

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活化的人外周血单核细胞中miR-19a、miR-20a与组织因子的表达

背景和目的:研究暴露于高糖(HG)和脂多糖(LPS)的外周血单个核细胞(PBMCs)中参与TF转录后调控的miR-19a和miR-20a的行为，并评估血管紧张素II在这一过程中的作用。方法:在暴露于高糖(HG, 50 mM)， LPS (100 ng/mL)和Olmesartan (10-6 M)(一种血管紧张素II型1受体拮抗剂)的PBMCs中，评估TF促凝活性(PCA，一阶段凝血试验)，抗原(Ag, ELISA)， miR-19a和miR-20a水平(特异性TaqMan®MicroRNA测定)。结果:与对照血糖(11.1 mM)相比，HG增加了TF表达，降低了两种miRs。在hg激活的PBMCs中，LPS刺激TF表达并下调miR-20a, OLM可以逆转这一作用(10-6 M);在CG和HG条件下，LPS均未改变miR-19a的表达。结论:miR-19a和miR-20a可被激活TF表达的炎症刺激抑制，且其反应因刺激的不同而不同;血管紧张素II可能参与了这一机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Thrombosis

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