Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety.

Karamallah Al-Yousuf, Carl A Webster, Grant N Wheeler, Francesca Baldelli Bombelli, Victoria Sherwood
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引用次数: 5

Abstract

The African clawed frog, Xenopus laevis, has been used as an efficient pre-clinical screening tool to predict drug safety during the early stages of the drug discovery process. X. laevis is a relatively inexpensive model that can be used in whole organism high-throughput assays whilst maintaining a high degree of homology to the higher vertebrate models often used in scientific research. Despite an ever-increasing volume of biomedical nanoparticles (NPs) in development, their unique physico-chemical properties challenge the use of standard toxicology assays. Here, we present a protocol that directly compares the sensitivity of X. laevis development as a tool to assess potential NP toxicity by observation of embryo phenotypic abnormalities/lethality after NP exposure, to in vitro cytotoxicity obtained using mammalian cell lines. In combination with conventional cytotoxicity assays, the X. laevis phenotypic assay provides accurate data to efficiently assess the safety of novel biomedical NPs. © 2017 by John Wiley & Sons, Inc.

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结合细胞毒性评估和非洲爪蟾表型异常测定作为纳米材料安全性的预测因子。
非洲爪蟾(Xenopus laevis)已被用作药物发现过程早期阶段预测药物安全性的有效临床前筛选工具。X. laevis是一种相对便宜的模型,可用于整个生物体的高通量分析,同时与科学研究中经常使用的高等脊椎动物模型保持高度同源性。尽管生物医学纳米颗粒(NPs)的开发量不断增加,但其独特的物理化学性质对标准毒理学分析的使用提出了挑战。在这里,我们提出了一个方案,直接比较X. laevis发育的敏感性,作为通过观察NP暴露后胚胎表型异常/致死性来评估潜在NP毒性的工具,与使用哺乳动物细胞系获得的体外细胞毒性。结合传统的细胞毒性试验,X. laevis表型试验为有效评估新型生物医学NPs的安全性提供了准确的数据。©2017 by John Wiley & Sons, Inc。
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