Simultaneous assessment of blood coagulation and hematocrit levels in dielectric blood coagulometry.

IF 1 4区医学 Q4 BIOPHYSICS Biorheology Pub Date : 2017-01-01 DOI:10.3233/BIR-16118

Yoshihito Hayashi, Marc-Aurèle Brun, Kenzo Machida, Seungmin Lee, Aya Murata, Shinji Omori, Hidetoshi Uchiyama, Yoshinori Inoue, Toshifumi Kudo, Takahiro Toyofuku, Masayuki Nagasawa, Isao Uchimura, Tomomasa Nakamura, Takeshi Muneta

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引用次数: 7

Abstract

Background: In a whole blood coagulation test, the concentration of any in vitro diagnostic agent in plasma is dependent on the hematocrit level but its impact on the test result is unknown.

Objective: The aim of this work was to clarify the effects of reagent concentration, particularly Ca2+, and to find a method for hematocrit estimation compatible with the coagulation test.

Methods: Whole blood coagulation tests by dielectric blood coagulometry (DBCM) and rotational thromboelastometry were performed with various concentrations of Ca2+ or on samples with different hematocrit levels. DBCM data from a previous clinical study of patients who underwent total knee arthroplasty were re-analyzed.

Results: Clear Ca2+ concentration and hematocrit level dependences of the characteristic times of blood coagulation were observed. Rouleau formation made hematocrit estimation difficult in DBCM, but use of permittivity at around 3 MHz made it possible. The re-analyzed clinical data showed a good correlation between permittivity at 3 MHz and hematocrit level (R2=0.83).

Conclusions: Changes in the hematocrit level may affect whole blood coagulation tests. DBCM has the potential to overcome this effect with some automated correction using results from simultaneous evaluations of the hematocrit level and blood coagulability.

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电介质血液凝固测定中血液凝固和红细胞压积水平的同时评估。

背景:在全血凝试验中，血浆中任何体外诊断试剂的浓度取决于红细胞压积水平，但其对试验结果的影响尚不清楚。目的:本工作的目的是澄清试剂浓度的影响，特别是Ca2+，并找到一种方法来估计红细胞压积相容凝血试验。方法:在不同浓度的Ca2+或不同红细胞比容水平的样品上，采用介电凝血仪(DBCM)和旋转血栓弹性仪进行全血凝固试验。重新分析先前接受全膝关节置换术患者的临床研究中的DBCM数据。结果:观察到明显的Ca2+浓度和红细胞压积水平对凝血特征时间的依赖性。Rouleau形成使得在DBCM中难以估计红细胞压积，但在3mhz左右使用介电常数使其成为可能。重新分析的临床数据显示，3 MHz介电常数与红细胞压积水平具有良好的相关性(R2=0.83)。结论:红细胞压积水平的改变可能影响全血凝血试验。DBCM有可能克服这种影响，通过使用同时评估红细胞比容水平和血液凝固性的结果进行一些自动校正。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.