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{"title":"Leptospira: Molecular Detection of Pathogenic Species in Natural Sources.","authors":"Brittney Beigel, Ashutosh Verma","doi":"10.1002/cpmc.43","DOIUrl":null,"url":null,"abstract":"<p><p>This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) for the presence of lipl32, a gene that is present only in pathogenic Leptospira spp. The gene target used in this assay is important in that it only detects pathogenic leptospires and not the saprophytic leptospires that may be present in environmental samples. © 2017 by John Wiley & Sons, Inc.</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.43","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/cpmc.43","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) for the presence of lipl32, a gene that is present only in pathogenic Leptospira spp. The gene target used in this assay is important in that it only detects pathogenic leptospires and not the saprophytic leptospires that may be present in environmental samples. © 2017 by John Wiley & Sons, Inc.
钩端螺旋体:天然来源致病性物种的分子检测。
本文描述了一种环境水中钩端螺旋体DNA的快速检测方法。总之，从水样中提取DNA，并通过基于taqman的实时定量聚合酶链反应(qPCR)检测lipl32的存在，该基因仅存在于致病性钩端螺旋体中。该检测中使用的基因靶点很重要，因为它仅检测致病性钩端螺旋体，而不检测可能存在于环境样品中的腐生钩端螺旋体。©2017 by John Wiley & Sons, Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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