Complete genome sequence of lytic bacteriophage RG-2014 that infects the multidrug resistant bacterium Delftia tsuruhatensis ARB-1.

Q3 Biochemistry, Genetics and Molecular Biology Standards in Genomic Sciences Pub Date : 2017-12-18 eCollection Date: 2017-01-01 DOI:10.1186/s40793-017-0290-y

Ananda Shankar Bhattacharjee, Amir Mohaghegh Motlagh, Eddie B Gilcrease, Md Imdadul Islam, Sherwood R Casjens, Ramesh Goel

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引用次数: 5

Abstract

A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium Delftia tsuruhatensis strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against Delftia tsuruhatensis strain ARB-1. The strain ARB-1 belongs to the Comamonadaceae family of the Betaproteobacteria class. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150 ± 9 PFU/cell, 10-min, <5-min, respectively. The phage was found to be a dsDNA virus belonging to the Podoviridae family. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G + C content of 59.9%. Significant similarities in gene homology and order were observed between Delftia phage RG-2014 and the E. coli phage N4 indicating that it is a member of the N4-like phage group.

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感染多药耐药细菌tsuruhatdelftia ARB-1的裂解噬菌体RG-2014的全基因组序列

从一个大型城市污水处理厂分离到一株裂解噬菌体RG-2014，该噬菌体感染形成多药耐药细菌特鲁哈特德尔菲特菌株ARB-1作为宿主的生物膜。分离到裂解噬菌体RG-2014，用于开发基于噬菌体的抗特鲁哈特代尔夫特菌株ARB-1的治疗方法。菌株ARB-1属于Betaproteobacteria纲的Comamonadaceae。RG-2014属Podoviridae科，其类型、爆发大小、潜伏时间和隐匿时间为150±9 PFU/细胞，10 min。它具有直径为85纳米的等距二十面体衣壳。对分离的噬菌体进行全基因组测序，全长73.8 kbp, G + C含量为59.9%。Delftia噬菌体RG-2014与大肠杆菌噬菌体N4在基因同源性和序列上有显著的相似性，表明其属于N4样噬菌体类群。

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