[Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices].

Prikladnaia biokhimiia i mikrobiologiia Pub Date : 2016-09-01

P S Timashev, K N Bardakova, N V Minaev, T S Demina, T A Mishchenko, E V Mitroshina, A A Akovantseva, A A Koroleva, D S Asyutin, L F Pimenova, N A Konovalov, T A Akopova, A B Solov'eva, I V Mukhina, M V Vedunova, B N Chichkov, V N Bagratashvili

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Abstract

Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

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[神经系统细胞与结构可生物降解的壳聚糖基水凝胶基质的相容性]。

以不饱和壳聚糖衍生物和甲基丙烯酸透明质酸为原料，采用双光子激光聚合制备了用于细胞培养的水凝胶基质。已经评估了基质的粘附性和毒性，并且基质已被证明与原代海马细胞培养具有良好的相容性。观察到神经系统细胞在水凝胶基质表面培养形成形态正常的神经网络。MTT还原酶活性测定结果显示，在基质上培养的游离海马细胞的代谢状态与对照组相似。因此，基于不饱和多糖衍生物的激光交联基质与神经系统分化细胞具有良好的相容性，在神经移植中具有很大的应用潜力。

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Prikladnaia biokhimiia i mikrobiologiia

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