INFLUENCE OF HYPOXIC PRECONDITIONING ON THE MECHANISMS OF BLOOD OXYGEN TRANSPORT AND OXIDATIVE DAMAGES DURING HEPATIC ISCHEMIA-REPERFUSION IN RABBITS.

Abstract

The effect of hypoxic preconditioning (HP) on the blood oxygen-binding properties and liver oxidative damages was determine during hepatic ischemia-reperfusion (HIR) in rabbits. Animals were randomized into two experimental groups: 1st (HIR) - hepatic ischemia (30 min by Pringle maneuver) and reperfusion (120 min); 2nd (HP+HIR) – before HIR the rabbits were passed through hypoxic chamber at 3500 m altitude during 1 hr/day (3 times day after day). The parameters of blood oxygen transport (р50, рСО2, рО2, рН, HCO3 -, ABE and ect.), lipid peroxidation products (conjugated dienes, Schiff bases) and blood hepatic markers (ALT, AST) were detected. It’s found that HIR leads to decline in hemoglobin oxygen affinity, activation of lipid peroxidation processes and elevation of ALT and AST activities in the 1st group. Hypoxic preconditioning (2nd group) markedly increased hemoglobin oxygen affinity, reduced the lipid peroxidation processes and ALT and AST activities in the blood during HIR. Thus, HP has a protective effect during HIR through elevation of hemoglobin oxygen affinity and declining hepatic oxidative damages.