Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)最新文献

We showed that a cortical maturation rhythms of people with hard-of-hearing is the subject to the same laws as the background rhythm persons normally hear: with age on the background EEG in the people with hard-of-hearing is a gradual increase in α-rhythm, but still normalized spectral power of the α- rhythm on the background's EEG of teenagers with hard-of-hearing remains below the similar indicators adolescents normally, and greater representation of slow waves. It was found that the background's EEG of teenagers with hard-of-hearing observed moving focus of α-occipital activity in the parietal cortex, which is not observed in the hard-of-hearing children. It can be associated with an increased activity in the limbic system on the background of bark tone in the hard-of-hearing children.

The results of γ-glutamyl carboxylase gene rs2592551 polymorphism determining in 170 patients with ischemic atherothrombotic stroke and 124 subjects without acute cerebrovascular disease (control group) have been evaluated. Obtained results revealed that rs2592551 polymorphism was related to ischemic stroke in Ukrainian population. The risk for this disease in patients with T/T genotype was higher than in major C-allele carriers (odds ratio (OR) = 3.117; 95% confidence interval (CI) = 1.016-9.566; P = 0.047). After dividing patients into subgroups, formed by the presence of certain risk factors for atherosclerosis, similar association has been established for women and non-smokers. At the same time, the heterozygous genotype (C/T) in females had significantly protective effect against ischemic stroke development when compared to C/C and T/T genotypes (OR = 0.460; 95% CI 0.213-0.994; P = 0.048). Statistical significance of these results persisted even after adjustment for age, body mass index, smoking and hypertension.

The influence of poly(ADP-ribose) polymerase (PARP) in- hibitor 4-hydroxyquinazoline (4-HQ) on the level of DNA damage and on the death of thymic and lymph node cells in mouse model of immune complex injury was investigated to reveal its possible cytoprotective effect. As shown by comet assay, DNA damage index of immune cells was increased 4,0 times in mice with immune complex-mediated pathology induced by a long-term immunization of CBA mice with bovine serum albumin (BSA), P<0,001. The percentage of thymic cells with strong DNA damage was increased to 77% under immunization (compared to 1,5% in control mice) and the percentage of such cells from lymph nodes was increased to 80% (compared to 0% in control), in both cases P< 0,001. Genotoxic stress was reduced by treatment of immunized mice with 4-HQ: the percentage of lymphocytes with strong DNA damage was significantly decreased that promoted increase in the amount of cells having intact DNA. PARP inhibition exerted a strong cytoprotective effect: viability of thymus and lymph node cells was increased mainly due to reduced level of necrosis. So, our results suggest that PARP may be involved in thymic and lymph node cell damage in immune complex mediated pathology and give evidence that inhibition of this enzyme may constitute a perspective target in immune complex diseases prevention and therapy.

The formation of connective tissue changes in the spinal canal of rats, caused by the method of intervertebral disc ruptures, in association with a mixture of epidural injection of longidaza and diprospan has been researched. Simultaneous injection of hialuronidase and betamethasone in composition of diprospan and longidaza, revealed a high synergistic effect and anti-fibrotic activity. Combined antifibrosis action of these two drugs is stronger than the sum of the actions of the two drugs in their separate use. Reducion of the maximum thickness of the fibrous layer was 49%, compared with the reference value and reduction of the total area of the fibrous formation was 46%. In our opinion, due to anti-inflammatory effects and the ability to reduce epidural scar adhesion, this complex is promising for its therapeutic use in treatment of spinal epidural fibrosis.

We studied the effect of curcumin on the cardiomyocytes viability, processes of oxidative phosphorylation in the mitochondria of cardiomyocytes, their pro- and antioxidant balance in doxorubicin-induced oxidative stress. It has been revealed that administration of doxorubicin to rats led to a significant increase in the secondary products of lipid peroxidation (TBARS) in mitochondria by 21 and H(2)0(2) by 76%, reduction of the enzymatic activity of mitochondrial Mn-SOD by 14% and intensified catalase activity by 80% compared with the control. After combined use of doxorubicin and curcumin the content of TBARS and H(2)0(2) increased by 14 and 26%, respectively, the enzymatic activity of catalase decreased by 28%, and mitochondrial Mn-SOD activity intensified by 9%. During the incubation with doxorubicin, the number of live cells decreased by 30.4% and the number of necrotic cells increased by 30.4% relative to control. Coadministration of doxorubicin and curcumin led to augmented cell viability by 8%, while the number of necrotic cells reduced by 8% compared with the use of doxorubicin only. In assessing the parameters of mitochondrial respiration in rats that received injections of doxorubicin active breathing index (V(3)) fell by 43.8%, the oxidation rate of the contingency of phosphorylation (V(3)/V(4)(ATp)) decreased by 47% and phosphorylation efficiency index (ADP/O) also declined by 31.7% respectively compared with the control. The combined use of doxorubicin and curcumin improved the indicators of mitochondrial respiration compared to using only doxorubicin: V(3) raised by 25%, V(3)/V(4)(ATP)by 18% and ADP/O by 12% respectively.

Experiments on rabbits with a model of acute temporary ocular hypertension created by intragastric administration of water (100 ml/kg) have been performed. It was found that intravenous administration or instillation into the eye of blocker of NMDA-receptor 1-adamantylethyloxy-3-morpholino-2- propanol hydrochloride (Ademol) unlike amantadine sulfate results in a significant decrease in intraocular pressure (IOP). It was also discovered that such ocular hypotensive effect takes place in animals with unchanged ophthalmotonus. Taking into account neuroretinoprotective and hypotensive ocular hypotensive properties of Ademol this drug appears to be perspective in the treatment of ischemic disorders of the retina and optic nerve, especially under the conditions of increased IOP.

In vivo assessment of bone mineral density (BMD) and bone mineral content (BMC) indices gives an objective description of the possible rates of bone loss during aging and various diseases, as well as response to treatment by various drugs, affecting bone metabolism. This study was conducted on 376 Wistar rats of both genders, divided into 12 age groups using the method of dual-energy X-ray absorptiometry. Studying the parameters of BMD and BMC depending on age and sex has found no bone mass opeak) in rats of both sexes. Bone mineral density indices were increased in both male and female rats during growing, more pronounced in males. These differences are noted in all age groups of animals. Moreover, we have established age and gender particularities of BMC indices in different parts of the skeleton. In female rats, a significant increase of BMD and BMC parameters have been detected in age from 1.5 to 2 months (33%), from 2 to 3 months - by 12.5%. In males, the increase of BMD indices was found to happen 1 month later and it was less pronounced (from 2 to 3 months - 27%, from 4 to 5 months - 5%). In other age periods, the dynamics of BMD indices had only an upward trend, but significant differences different groups were not established. Our results of BMD and BMC indices and their age and gender features can be used for further research in osteology as reference data for modeling of experimental osteoporosis in rats and study of response to the use of different antiosteoporotic drugs.

It was shown the alterations in hydrogen sulfide (H(2)S) metabolism and the development of oxidative and nitrozative stress in cardiovascular system by aging. The administration of pyridoxal-5-phosphate as cofactor of H(2)S synthesizing enzymes restored endogenous H(2)S level and redox state in the heart and aorta tissues. Under these conditions, the following indicators of oxidative stress were significantly decreased in heart and aorta tissues: superoxide generation rate (·0(2)(-)) and hydroxyl (·OH) anion radicals, compared with significantly elevated levels of these parameters in old animals. We also found the reduction of non-enzymatic (diene conjugates and malonic dialdehyde) and enzymatic (uric acid, LTC(4) and TxB(2)) lipid oxidation products levels in old rats under H(2)S synthesis stimulation that confirms the restriction of oxidative stress. An important consequence of endogenous synthesis stimulation of hydrogen sulfide during aging is a decrease of nitrozative stress, such as iNOS activity and nitrate reductase, as well as recovery of constitutive NO synthase activity, indicating the importance of this gas transmitter in cardiovascular system. Thus, stimulation of hydrogen sulfide endogenous synthesis contributed to reduced production of reactive oxygen species (oxidative stress) and nitrogen (nitrozative stress) in heart and aorta tissues with aging. The presence of a pronounced antioxidant effect and modulating influence of pyridoxal-5- phosphate in the redox state of heart tissue and blood vessels during aging suggests cardioprotective properties of the substance and prospects for future research.

We determined the changes of time, amplitude and derivatives of these indicators of rheovasograms of shin in sportsmen of youth age and high level of skills engaged in volleyball, athletics and wrestling with sports experience at least 3 years. We determined significant differences in the value of time, amplitude and derivatives of these indicators ofrheovazograms of shin in males youth age, depending on the impact of intense exercise. The volleyball players compared to youth who do not exercise, reliably large values of the overall tone of the arteries (11,2%), arteries of large diameter (8,2%), arteries of medium and small diameters (13,5%), time ascending part of rheovazograms (2%) and a slow passage (5,9%) and less dycrotycs index (17%), duration ofrheographic wave (3,7%), time descending part of rheovazograms (10%). The persons of control group had higher values of baseline impedance than wrestlers (9,9%) and athletes (13,7%) and all amplitude indicators rheograms of the shin than athletes (average 12-15%) and wrestlers (22-23%). The size and volume of blood flow to the arteries of the extremities adapted to the metabolic needs of the relevant muscles. In volleyball players compared to the athletes, a lower duration of rheographic wave (10%), time of downlink part of the rheograms (12,7%), the base impedance (17,2%), amplitude of the systolic wave (17%) and rapid blood filling (21%), all indicators tone of arteries (12,2-16,9%) and greater value of time of rising part of rheograms (3,5%) and slow blood filling (5,9%). Wrestlers compared to the athletes have a lower duration of rheographic wave (6,1%), time of downlink part of the rheograms (6,1%), the amplitude of the systolic wave (9,3%), compared with volleyball players set lower values of average speed of fast blood filling (15,6%), tone of arteries with different diameters (15-16,5%), all amplitude indicators (20-28%), time slow blood filling (9,7%), ascending parts rheogram (10,3%). Thus, the level ofphysical activity and especially muscle activity has a significant effect on the performance of regional blood flow.

The functional changes of retina morphological parameters of macular zone were investigated under condition of long term action of fenofibrate, 145 mg in patients with diabetes mellitus type 2. Dynamic observation of certain ophthalmologic (visual acuity, macular thickness zone) and system functional parameters (lipid fractions content in serum) in long-term use of this medical drug in 20 patients with diabetes type 2 and DMO for 8 months. In this period we revealed some positive changes in the functional state of the macular area: increase of visual acuity, decrease of macula thickness and the improvement of the lipid profile in blood serum.