Recovery time course of erythrocyte deformability following exposure to shear is dependent upon conditioning shear stress.

IF 1 4区 医学 Q4 BIOPHYSICS Biorheology Pub Date : 2018-01-01 DOI:10.3233/BIR-17151
Lennart Kuck, Marijke Grau, Michael J Simmonds
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引用次数: 15

Abstract

Background: Red blood cell (RBC) deformability may increase, or decrease, following application of shear stress ("shear conditioning"), depending upon the specific magnitude and duration of exposure. However, the time course of altered RBC deformability following shear remains unresolved.

Objective: We utilised shear conditioning known to increase (10 Pa) or decrease (64 Pa) RBC deformability and subsequently rested the cells; serial measurements of deformability during the rest period facilitated defining the time course of recoverability. A second experiment repeated the shear conditioning and recovery period to explore whether multiple duty-cycles augmented the response following the initial exposure.

Methods: Shear conditioning was performed for 300 s at the desired shear stress. Ektacytometry was used to quantify human RBC deformability immediately and during rest (3, 5, 60, 120, 240, 300 s) using discrete samples. RBC were shear conditioned twice in a separate experiment, with 300 s rest separating the conditioning.

Results: Shear conditioning at 10 Pa induced increased cell deformability by 19.5 ± 0.3%, which reduced to 7.2 ± 0.4% after 300 s of rest. Shear conditioning at 64 Pa decreased cell deformability by 30.5 ± 13.9%, and after 300 s rest, remained decreased (19.3 ± 9.4%) compared with baseline. The second duty-cycle augmented initial responses induced by shear conditioning.

Conclusion: Specific shear conditioning results in either temporarily increased cell deformability, or a less reversible decrease of RBC deformability.

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红细胞受剪切后变形能力的恢复时间过程取决于条件剪应力。
背景:在施加剪切应力(“剪切调节”)后,红细胞(RBC)的变形能力可能增加或减少,这取决于暴露的具体幅度和持续时间。然而,剪切后红细胞变形能力改变的时间过程仍未确定。目的:我们利用已知的剪切条件增加(10pa)或减少(64pa)红细胞变形能力,随后休息细胞;在休息期间对变形能力的连续测量有助于确定可恢复性的时间过程。第二个实验重复了剪切条件和恢复期,以探索多重占空比是否增强了初始暴露后的响应。方法:在所需剪切应力下进行剪切调理300 s。使用离散样本,使用Ektacytometry定量人红细胞立即和休息时(3,5,60,120,240,300 s)的变形能力。在单独的实验中,RBC被剪切两次,间隔300 s。结果:在10 Pa剪切条件下,细胞变形率提高19.5±0.3%,静置300 s后,细胞变形率降低至7.2±0.4%。在64 Pa剪切条件下,细胞变形能力降低了30.5±13.9%,休息300 s后,与基线相比,细胞变形能力仍然降低(19.3±9.4%)。剪切条件引起的二次占空比增强初始响应。结论:特异性剪切调节可使红细胞变形能力暂时增加,或使红细胞变形能力不可逆地降低。
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来源期刊
Biorheology
Biorheology 医学-工程:生物医学
CiteScore
2.00
自引率
0.00%
发文量
5
审稿时长
>12 weeks
期刊介绍: Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.
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