Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma.

Q2 Biochemistry, Genetics and Molecular Biology Cell Communication and Adhesion Pub Date : 2018-12-01 DOI:10.1080/15419061.2018.1464000

Jing Kong, Han Zhao, Qianhui Shang, Zhifei Ma, Ni Kang, Junling Tan, Hyat Ahmed Ibrahim Alraimi, Tingjiao Liu

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引用次数: 9

Abstract

Salivary gland adenoid cystic carcinoma (SACC) is one of the most common malignancies in the oral and maxillofacial region. Carcinoma-associated fibroblast (CAF) is an important component in the tumor microenvironment and participates in SACC progression. In this study, we established a CAF cell line derived from a human SACC and named it CAF-SA. It was identified that CAF-SA expressed typical CAF biomarkers. Then, we studied the cellular communications between CAF-SA, tumor cells and endothelial cells. It was found that CAF-SA promoted the migration, invasion, and proliferation of SACC tumor cells in vitro. In addition, tube formation by endothelial cells was enhanced by CAF-SA. In vivo experiment showed that SACC cells formed larger xenografts in nude mice when they were transplanted with CAF-SA. Overall, we demonstrated that CAF-SA exhibited the most important defining feature of CAF by promoting cancer progression.

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来源于人唾液腺腺样囊性癌的癌相关成纤维细胞系的建立和表征。

涎腺腺样囊性癌是口腔颌面部最常见的恶性肿瘤之一。癌相关成纤维细胞(CAF)是肿瘤微环境的重要组成部分，参与SACC的进展。在本研究中，我们从人SACC中提取了一株CAF细胞系，并将其命名为CAF- sa。鉴定出CAF- sa表达典型的CAF生物标志物。然后，我们研究了ca - sa与肿瘤细胞和内皮细胞之间的细胞通讯。体外实验发现，cafa - sa能促进SACC肿瘤细胞的迁移、侵袭和增殖。此外，ca - sa可促进内皮细胞形成小管。体内实验表明，用ca - sa移植的SACC细胞在裸鼠体内形成较大的异种移植物。总的来说，我们证明了CAF- sa通过促进癌症进展表现出CAF最重要的决定性特征。

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来源期刊

Cell Communication and Adhesion 生物-生化与分子生物学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cessation Cell Communication and Adhesion is an international Open Access journal which provides a central forum for research on mechanisms underlying cellular signalling and adhesion. The journal provides a single source of information concerning all forms of cellular communication, cell junctions, adhesion molecules and families of receptors from diverse biological systems. The journal welcomes submission of original research articles, reviews, short communications and conference reports.