Studies on the Dual Cytotoxicity and Antioxidant Properties of Berberis vulgaris Extracts and Its Main Constituent Berberine.

Q1 Pharmacology, Toxicology and Pharmaceutics Advances in Pharmacological Sciences Pub Date : 2018-01-08 eCollection Date: 2018-01-01 DOI:10.1155/2018/3018498

Lamyae El Khalki, Mounir Tilaoui, Abdeslam Jaafari, Hassan Ait Mouse, Abdelmajid Zyad

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引用次数: 24

Abstract

The present study attempts to investigate the cytotoxic activity of ethanol and ethyl acetate extracts of the Moroccan Berberis vulgaris and its major component berberine, together with exploring their antioxidant properties. It also consists of studying the combination effect of berberine and S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, against the human breast adenocarcinoma cell line (MCF-7). Using the MTT assay, we report a differential cytotoxic effect of ethanol and ethyl acetate extracts since the ethanol extract is more cytotoxic than the ethyl acetate one, with IC₅₀ = 3.54 μg/mL and 596.71 μg/mL, respectively. Interestingly, no cytotoxic effect was observed against normal cells. Furthermore, these extracts showed a remarkable antioxidant activity as measured by the DPPH free radicals scavenging assay. In fact, the IC₅₀ values are 69.65 μg/mL and 77.75 μg/mL for the ethanol and ethyl acetate extracts, respectively. In addition, several concentrations of berberine, when combined with the NO donor used at IC₃₀, induced a synergistic cytotoxic activity at concentrations ranging from 8.40 μM to 33.60 μM, as revealed by the combination index values, using the Chou-Talalay method. However, at the other concentrations tested, an antagonistic effect was observed. The observed cytotoxicity was related to apoptosis induction as demonstrated by the annexin-V-streptavidin FITC-staining analysis.

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小檗提取物及其主要成分小檗碱的双重细胞毒性和抗氧化性研究。

本研究旨在研究摩洛哥小檗的乙醇和乙酸乙酯提取物及其主要成分小檗碱的细胞毒活性，并探讨其抗氧化性能。研究小檗碱与一氧化氮(NO)供体s -亚硝基-n -乙酰青霉胺(SNAP)联合抗人乳腺腺癌细胞(MCF-7)的作用。利用MTT法，我们报告了乙醇提取物和乙酸乙酯提取物的细胞毒性差异，因为乙醇提取物比乙酸乙酯提取物更具有细胞毒性，IC50分别为3.54 μg/mL和596.71 μg/mL。有趣的是，没有观察到对正常细胞的细胞毒性作用。此外，通过DPPH自由基清除实验，这些提取物显示出显著的抗氧化活性。乙醇和乙酸乙酯提取物的IC50值分别为69.65 μg/mL和77.75 μg/mL。此外，用Chou-Talalay法测定了几种浓度的小檗碱与一氧化氮供体在IC30下联合使用时，在8.40 μM ~ 33.60 μM范围内具有协同细胞毒活性。然而，在其他浓度的测试中，观察到拮抗作用。annexin-V-streptavidin fitc染色分析表明，细胞毒性与诱导凋亡有关。

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来源期刊

Advances in Pharmacological Sciences PHARMACOLOGY & PHARMACY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

14 weeks

期刊介绍： Advances in Pharmacological and Pharmaceutical Sciences is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of experimental and clinical pharmacology, pharmaceutics, medicinal chemistry and drug delivery. Topics covered by the journal include, but are not limited to: -Biochemical pharmacology, drug mechanism of action, pharmacodynamics, pharmacogenetics, pharmacokinetics, and toxicology. -The design and preparation of new drugs, and their safety and efficacy in humans, including descriptions of drug dosage forms. -All areas of medicinal chemistry, such as drug discovery, design and synthesis. -Basic biology of drug and gene delivery through to application and development of these principles, through therapeutic delivery and targeting. Areas covered include bioavailability, controlled release, microcapsules, novel drug delivery systems, personalized drug delivery, and techniques for passing biological barriers.