Quercetin inhibits LPS-induced macrophage migration by suppressing the iNOS/FAK/paxillin pathway and modulating the cytoskeleton.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2019-12-01 Epub Date: 2018-08-01 DOI:10.1080/19336918.2018.1486142

Shuna Cui, Qingqing Wu, Juan Wang, Min Li, Jing Qian, Shihua Li

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引用次数: 49

Abstract

ABSTRACT The natural flavonoid quercetin has antioxidant, anti-inflammatory, and anticancer effects. We investigated the effect of quercetin on lipopolysaccharide (LPS)-induced macrophage migration. Quercetin significantly attenuated LPS-induced inducible nitric oxide synthase (iNOS)-derived nitric oxide (NO) production in RAW264.7 cells without affecting their viability. Additionally, quercetin altered the cell size and induced an elongated morphology and enlarged the vacuoles and concentrated nuclei. Quercetin significantly disrupted the F-actin cytoskeleton structure. Furthermore, quercetin strongly inhibited LPS-induced macrophage adhesion and migration in a dose-dependent manner. Moreover, quercetin inhibited the LPS-induced expression of p-FAK, p-paxillin, FAK, and paxillin as well as the cytoskeletal adapter proteins vinculin and Tensin-2. Therefore, quercetin suppresses LPS-induced migration by inhibiting NO production, disrupting the F-actin cytoskeleton, and suppressing the FAK–paxillin pathway. Quercetin may thus have potential as a therapeutic agent for chronic inflammatory diseases.

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槲皮素通过抑制iNOS/FAK/paxillin通路和调节细胞骨架抑制lps诱导的巨噬细胞迁移。

天然类黄酮槲皮素具有抗氧化、抗炎和抗癌作用。我们研究了槲皮素对脂多糖(LPS)诱导巨噬细胞迁移的影响。槲皮素显著减弱lps诱导的一氧化氮合酶(iNOS)衍生的一氧化氮(NO)在RAW264.7细胞中的产生，但不影响细胞的生存能力。槲皮素还能改变细胞大小，使细胞形态变长，液泡增大，细胞核集中。槲皮素明显破坏了f -肌动蛋白的细胞骨架结构。槲皮素对lps诱导的巨噬细胞粘附和迁移具有剂量依赖性。此外，槲皮素抑制lps诱导的p-FAK、p-paxillin、FAK和paxillin以及细胞骨架连接蛋白vinculin和Tensin-2的表达。因此，槲皮素通过抑制NO生成、破坏f -肌动蛋白细胞骨架和抑制FAK-paxillin通路来抑制lps诱导的迁移。槲皮素可能因此有潜力作为慢性炎症性疾病的治疗剂。

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