Lei Ji, Liping Chen, Xiaofang Wu, Deshun Xu, Jiankang Han
{"title":"[Complete Genome Analysis of a Recombinant GI Norovirus Identified in Huzhou,China].","authors":"Lei Ji, Liping Chen, Xiaofang Wu, Deshun Xu, Jiankang Han","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>To study the genotype and genomic and molecular organization of a GI norovirus isolate from Huzhou, China, the genomic sequence of 2008/Huzhou/N11 was amplified by RT-PCR, then the PCR product was purified,sequenced, and input into phylogenetic and Simplot analyses. The complete genomic sequence of the 2008/Huzhou/N11 strain was 7691nucleotides (nt) long. It contained three open reading frames(ORFs):ORF1,ORF2,and ORF3,with lengths of 5367,1623,and 630 nt, respectively. Phylogenetic analysis revealed that the RdRp region of 2008/Huzhou/N11 belonged to genotype GI.2,and the VP1 and VP2regions belonged to genotype GI.6.The SimPlot analysis indicated that potential recombination points in 2008/Huzhou/N11 were located upstream of the ORF1/ORF2 overlap. The complete genomic sequence of this recombinant GI.2/GI.6 strain can serve as a reference sequence for the phylogenetic analysis of norovirus evolution.</p>","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 3","pages":"263-6"},"PeriodicalIF":0.0000,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bing du xue bao = Chinese journal of virology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
To study the genotype and genomic and molecular organization of a GI norovirus isolate from Huzhou, China, the genomic sequence of 2008/Huzhou/N11 was amplified by RT-PCR, then the PCR product was purified,sequenced, and input into phylogenetic and Simplot analyses. The complete genomic sequence of the 2008/Huzhou/N11 strain was 7691nucleotides (nt) long. It contained three open reading frames(ORFs):ORF1,ORF2,and ORF3,with lengths of 5367,1623,and 630 nt, respectively. Phylogenetic analysis revealed that the RdRp region of 2008/Huzhou/N11 belonged to genotype GI.2,and the VP1 and VP2regions belonged to genotype GI.6.The SimPlot analysis indicated that potential recombination points in 2008/Huzhou/N11 were located upstream of the ORF1/ORF2 overlap. The complete genomic sequence of this recombinant GI.2/GI.6 strain can serve as a reference sequence for the phylogenetic analysis of norovirus evolution.
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