An Overview of Digital PCR.

Abstract

Digital polymerase chain reaction (dPCR) is a new method for absolute quantification of nucleic acids. The dPCR reaction solution is divided into numerous partitions followed by independent amplification. Target copy number is counted using statistical analyses of positive signals. in contrast to quantitative PCR, a standard curve is not necessary for dPCR. Here, we reviewed the development, principles, and applications of dPCR.