{"title":"[Endocytosis pathway and intracellular distribution of heparosan].","authors":"Huan-huan Peng, Ying Li, Jia-yi Yuan, Jing-xiao Chen, Jing-hua Chen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, the endocytosis pathway of heparosan and its intracellular distribution were\ninvestigated in MCF-7 tumor cells and COS7 normal cells. The endocytosis inhibition and cellular probe\nlocation experiments showed that MCF-7 tumor cells took heparosan more efficiently and selectively than COS7\ncells. The cellular uptake of heparosan was energy-dependent in both MCF-7 tumor cells and COS7 normal\ncells. Moreover, the major endocytosis pathway of heparosan into MCF-7 tumor cells was caveolin-mediated\nendocytosis and macropinocytosis. The internalized heparosan was mainly located in lysosomes of the cells.</p>","PeriodicalId":35924,"journal":{"name":"药学学报","volume":"52 3","pages":"474-80"},"PeriodicalIF":0.0000,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"药学学报","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, the endocytosis pathway of heparosan and its intracellular distribution were
investigated in MCF-7 tumor cells and COS7 normal cells. The endocytosis inhibition and cellular probe
location experiments showed that MCF-7 tumor cells took heparosan more efficiently and selectively than COS7
cells. The cellular uptake of heparosan was energy-dependent in both MCF-7 tumor cells and COS7 normal
cells. Moreover, the major endocytosis pathway of heparosan into MCF-7 tumor cells was caveolin-mediated
endocytosis and macropinocytosis. The internalized heparosan was mainly located in lysosomes of the cells.
药学学报Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍：
Acta Pharmaceutica Sinica B (APSB) is a bimonthly English peer-reviewed online journal in ScienceDirect, which publishes significant original research articles, communications and high quality reviews of recent advances. APSB encourages submissions from all areas of pharmaceutical sciences, including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics.
APSB is a part of the series Acta Pharmaceutica Sinica, which was founded in 1953. The journal is co-published by Elsevier B.V., in association with the Institute of MateriaMedica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association.
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