[Canine Parvovirusin Diarrheal Dogs and Analyses of the Full-Length VP2 Gene of Dogs].

Xuefeng Cao, Guangneng Peng, Xiangming Huang, Wenbo Wang, Yinan Tian, Xiaoyang Xu, Shuangshuang Lei, Yanyi Yang, Chao Gong, Kuixing Yang, Bo Yuan, Zhihua Ren, Zhijun Zhong
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Abstract

We investigated infection by canine parvovirus and genetic variation of the VP2 gene. We collected feces samples of 50 diarrheal dogs in Sichuan Province, China. Analyses polymerase chain reaction (PCRs), agarose gel electrophoresis, and amplification of the complete sequence of canine parvovirus were done. We observed 19PCR-positive samples. Sequencing analyses of 15PCR-positive samples based on amplification of the complete VP2 gene showed all to be CPV-2a,and to be polymerized with Sichuan isolates. These results suggest that the common epidemic strain in Sichuan Province is CPV-2a,and may originate from the same strain. Compared with reference strains, there were no significant variations in canine parvovirus in Sichuan Province, China.

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犬细小病毒腹泻犬及其VP2全长基因分析[j]。
我们研究了犬细小病毒感染和VP2基因的遗传变异。我们收集了中国四川省50只腹泻犬的粪便样本。对犬细小病毒进行了聚合酶链反应(pcr)、琼脂糖凝胶电泳和全序列扩增分析。我们观察到19份pcr阳性样本。通过VP2全基因扩增,对15份pcr阳性样本进行测序分析,结果均为CPV-2a,并与四川分离株聚合。上述结果提示,四川省常见的流行毒株为CPV-2a,可能来源于同一毒株。与参比株相比,犬细小病毒在四川地区无明显差异。
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