Concordance between Immunohistochemistry and Microarray Gene Expression Profiling for Estrogen Receptor, Progesterone Receptor, and HER2 Receptor Statuses in Breast Cancer Patients in Lebanon.

IF 1.6 Q4 ONCOLOGY International Journal of Breast Cancer Pub Date : 2018-05-31 eCollection Date: 2018-01-01 DOI:10.1155/2018/8530318
Ghina B Fakhri, Reem S Akel, Maya K Khalil, Deborah A Mukherji, Fouad I Boulos, Arafat H Tfayli
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引用次数: 5

Abstract

Introduction: Accurate evaluation of estrogen and progesterone receptors and HER2 is critical when diagnosing invasive breast cancer for optimal treatment. The current evaluation method is via immunohistochemistry (IHC). In this paper, we compared results of ER, PR, and HER2 from microarray gene expression to IHC in 81 fresh breast cancer specimens.

Methods: Gene expression profiling was performed using the GeneChip Human Genome U133 Plus 2.0 arrays (Affymetrix Inc). Immunohistochemical staining for estrogen receptor, progesterone receptor, and HER2 status was performed using standard methods at a CAP-accredited pathology laboratory. Concordance rates, agreement measures, and kappa scores were calculated for both methods.

Results: For ER, Kappa score was 0.918 (95% CI, 0.77.3-1.000) and concordance rate was 97.5% (95% CI, 91.4%-99.7%). For PR, Kappa score was 0.652 (95% CI, 0.405-0.849) and concordance rate was 86.4% (95% CI, 77%-93%). For HER2, Kappa score was 0.709 (95% CI, 0.428-0.916) and concordance rate was 97.5% (95% CI, 91.4%-99.7%).

Conclusion: Our results are in line with the available evidence with the concordance rate being the lowest for the progesterone receptor. In general, microarray gene expression and IHC proved to have high concordance rates. Several factors can increase the discordance rate such as differences in sample processing.

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黎巴嫩乳腺癌患者雌激素受体、孕激素受体和HER2受体状态的免疫组织化学和微阵列基因表达谱的一致性
简介:准确评估雌激素和孕激素受体和HER2在诊断浸润性乳腺癌以获得最佳治疗时至关重要。目前的评估方法是通过免疫组织化学(IHC)。在本文中,我们比较了81例新鲜乳腺癌标本中从微阵列基因表达到IHC的ER、PR和HER2的结果。方法:采用GeneChip Human Genome U133 Plus 2.0阵列(Affymetrix Inc .)进行基因表达谱分析。在cap认可的病理实验室使用标准方法进行雌激素受体、孕激素受体和HER2状态的免疫组织化学染色。计算两种方法的一致性率、一致性测量和kappa评分。结果:ER Kappa评分为0.918 (95% CI, 0.77.3 ~ 1.000),符合率为97.5% (95% CI, 91.4% ~ 99.7%)。PR的Kappa评分为0.652 (95% CI, 0.405 ~ 0.849),一致性率为86.4% (95% CI, 77% ~ 93%)。对于HER2, Kappa评分为0.709 (95% CI, 0.428-0.916),一致性率为97.5% (95% CI, 91.4%-99.7%)。结论:我们的结果与已有证据一致,黄体酮受体的符合率最低。总的来说,微阵列基因表达和免疫组化具有很高的一致性。有几个因素可以增加不一致率,如样品处理的差异。
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来源期刊
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: International Journal of Breast Cancer is a peer-reviewed, Open Access journal that provides a forum for scientists, clinicians, and health care professionals working in breast cancer research and management. The journal publishes original research articles, review articles, and clinical studies related to molecular pathology, genomics, genetic predisposition, screening and diagnosis, disease markers, drug sensitivity and resistance, as well as novel therapies, with a specific focus on molecular targeted agents and immune therapies.
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