In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function

Shannon G. Loelius, Sherry L. Spinelli, Katie L. Lannan, Richard P. Phipps
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引用次数: 2

Abstract

In this document, we describe methods for the isolation, treatment, and functional testing of human blood platelets in vitro. Functional assays for inflammatory function include flow cytometry and immunoassays for platelet release of platelet factor 4, soluble CD40L, prostaglandin E2, and thromboxane. Assays for platelet hemostatic function described here examine platelet spreading, aggregation using platelet-rich plasma, and thromboelastography. Also described here are methods for testing cigarette smoke on primary human platelets in vitro, which our lab developed to address a major knowledge gap regarding how cigarette smoke dysregulates platelets and how this platelet dysregulation contributes to cardiovascular disease. Some of these protocols may be repurposed for investigation of the toxicity potential of other tobacco products and environmental insults. © 2018 by John Wiley & Sons, Inc.

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烟草和非烟草制品对人血小板功能影响的体外方法研究
在这篇文章中,我们描述了人类血小板体外分离、处理和功能测试的方法。炎性功能的功能测定包括流式细胞术和血小板因子4、可溶性CD40L、前列腺素E2和血栓素释放的免疫测定。本文所述的血小板止血功能试验检查血小板扩散、富血小板血浆聚集和血栓弹性成像。本文还介绍了在体外测试香烟烟雾对人类初级血小板的影响的方法,我们的实验室开发了这些方法,以解决关于香烟烟雾如何失调血小板以及血小板失调如何导致心血管疾病的主要知识空白。其中一些议定书可重新用于调查其他烟草制品的潜在毒性和对环境的损害。©2018 by John Wiley &儿子,Inc。
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