Critical Assessment of the mdx Mouse with Ex Vivo Eccentric Contraction of the Diaphragm Muscle

Q1 Agricultural and Biological Sciences Current protocols in mouse biology Pub Date : 2018-08-14 DOI:10.1002/cpmo.49

Gregory C. Addicks, Phillip Marshall, Bernard J. Jasmin, Jean-Marc Renaud, Hongbo Zhang, Keir J. Menzies

引用次数: 1

Abstract

Muscle function and health progressively deteriorate during the progression of muscle dystrophies. The ability to objectively characterize muscle function and muscle damage is useful not only when comparing variants of dystrophy models, but also for characterizing the effects of interventions aiming to improve or halt the progressive decline of muscle function and muscle health. The protocols in this chapter describe the use of ex vivo eccentric contraction of the diaphragm muscle as a measure of muscle susceptibility to damage. Because muscle has a robust regenerative capacity, unhealthy muscle may be functionally close to normal; therefore, protocols for ex vivo characterization of muscle are often essential for assessing the effects of interventions. Additional methods that can be applied for assessment of dystrophic muscle are also highlighted. © 2018 by John Wiley & Sons, Inc.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

mdx小鼠离体膈肌偏心收缩的临界评估

在肌肉营养不良的过程中，肌肉功能和健康逐渐恶化。客观表征肌肉功能和肌肉损伤的能力不仅在比较营养不良模型的变体时很有用，而且对于表征旨在改善或停止肌肉功能和肌肉健康的进行性衰退的干预措施的效果也很有用。本章的方案描述了使用离体膈肌偏心收缩作为肌肉对损伤易感性的测量。由于肌肉具有强大的再生能力，不健康的肌肉在功能上可能接近正常;因此，对于评估干预措施的效果，肌肉的离体表征方案通常是必不可少的。另外的方法，可以应用于评估营养不良的肌肉也强调。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

自引率

0.00%

发文量

期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.