Augmentation of hepatoprotective potential of Aegle marmelos in combination with piperine in carbon tetrachloride model in wistar rats.

Q1 Chemistry Chemistry Central Journal Pub Date : 2018-08-20 DOI:10.1186/s13065-018-0463-9
Deepti Rathee, Anjoo Kamboj, Shabir Sidhu
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引用次数: 15

Abstract

The current study investigated hepatoprotective and antioxidant effects of Aegle marmelos leaves extract. The major constituent present in the extract i.e. rutin was quantified by using HPLC. Further, the study explored hepatoprotective effect of A. marmelos (70% ethanol extract) in combination with piperine. The normal control and carbon tetrachloride (CCl4) administered rats were divided into 7 groups. Hepatic damage biomarkers were determined in serum samples and oxidative stress biomarkers (malondialdehyde, reduced glutathione, glutathione reductase, glutathione peroxidase, glutathione-S-transferase, superoxide dismutase and catalase), pro-inflammatory and anti-inflammatory cytokines were determined in liver homogenates. CCl4 caused marked liver damage as evident by significant increased activities of serum alkaline phosphatase, bilirubin, lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, Interleukin 10 and Tumor necrosis factor-α levels compared to normal control. The oxidative stress parameters also significantly modulated in CCl4 group as compared to normal control. Treatment with A. marmelos reduced the severity of toxicity in a dose dependent fashion and the results of A. marmelos extract 50 mg/kg group were comparable to silymarin group. The low dose of A. marmelos extract (25 mg/kg) per se did not significantly reversed the hepatotoxicity but low dose of A. marmelos in combination with piperine showed significant reversal of hepatotoxicity. In conclusion, A. marmelos exerts potential hepatoprotective activity through its antioxidant and anti-inflammatory properties which was enhanced by co-treatment with piperine.

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甜瓜联合胡椒碱对四氯化碳模型wistar大鼠肝保护作用的增强。
本研究主要研究甜瓜叶提取物对肝脏的保护作用和抗氧化作用。采用高效液相色谱法测定了芦丁提取物的主要成分。进一步研究了甜瓜(70%乙醇提取物)与胡椒碱联合使用对肝脏的保护作用。将正常对照组和四氯化碳(CCl4)给药大鼠分为7组。测定血清样品中的肝损伤生物标志物,测定肝脏匀浆中的氧化应激生物标志物(丙二醛、还原性谷胱甘肽、谷胱甘肽还原酶、谷胱甘肽过氧化物酶、谷胱甘肽s转移酶、超氧化物歧化酶和过氧化氢酶)、促炎和抗炎细胞因子。血清碱性磷酸酶、胆红素、乳酸脱氢酶、丙氨酸转氨酶、天冬氨酸转氨酶、白细胞介素10和肿瘤坏死因子-α的活性显著高于正常对照组。与正常对照组相比,CCl4组氧化应激参数也有显著调节。蜜瓜提取物50 mg/kg组与水飞蓟素组的结果相当,以剂量依赖性方式降低了毒性严重程度。小剂量甜瓜提取物(25 mg/kg)本身没有显著逆转肝毒性,但小剂量甜瓜与胡椒碱联合使用有显著逆转肝毒性。综上所述,甜瓜具有抗氧化和抗炎作用,与胡椒碱共处理可增强其抗氧化和抗炎作用。
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来源期刊
Chemistry Central Journal
Chemistry Central Journal 化学-化学综合
CiteScore
4.40
自引率
0.00%
发文量
0
审稿时长
3.5 months
期刊介绍: BMC Chemistry is an open access, peer reviewed journal that considers all articles in the broad field of chemistry, including research on fundamental concepts, new developments and the application of chemical sciences to broad range of research fields, industry, and other disciplines. It provides an inclusive platform for the dissemination and discussion of chemistry to aid the advancement of all areas of research. Sections: -Analytical Chemistry -Organic Chemistry -Environmental and Energy Chemistry -Agricultural and Food Chemistry -Inorganic Chemistry -Medicinal Chemistry -Physical Chemistry -Materials and Macromolecular Chemistry -Green and Sustainable Chemistry
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Design, synthesis and biological potential of heterocyclic benzoxazole scaffolds as promising antimicrobial and anticancer agents. Regio and stereoselective synthesis of anticancer spirooxindolopyrrolidine embedded piperidone heterocyclic hybrids derived from one-pot cascade protocol. Augmentation of hepatoprotective potential of Aegle marmelos in combination with piperine in carbon tetrachloride model in wistar rats. Polyaniline/palladium nanohybrids for moisture and hydrogen detection. Benzoxazole derivatives: design, synthesis and biological evaluation.
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